Biochemical and Immunohistochemical Characterization of the Amyloid in Canine Amyloid-Producing Odontogenic Tumor
Biochemical and Immunohistochemical Characterization of the Amyloid in Canine Amyloid-Producing Odontogenic Tumor
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DOI：
10.1177/0300985810375047
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发表时间：
2010-09-01
影响因子：
2.4
通讯作者：
Taniyama, H.
中科院分区：
文献类型：
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作者：
Hirayama, K.;Miyasho, T.;Taniyama, H.
对犬产生淀粉样蛋白的牙源性肿瘤（APOT）的淀粉样蛋白进行生化和免疫组织化学评估。从犬 APOT 中纯化的淀粉样蛋白的 N 端氨基酸序列与大鼠成釉细胞和猪鞘蛋白中的序列惊人地相似。免疫组织化学显示，9只狗的APOT中的淀粉样蛋白与抗大鼠成釉细胞、抗猪鞘蛋白和抗犬APOT淀粉样蛋白有强烈反应，与抗猪牙釉蛋白有弱反应，但对细胞角蛋白、波形蛋白、结蛋白、α-平滑肌肌动蛋白、淀粉样蛋白A、胶质纤维酸性蛋白或S100蛋白的抗体呈阴性。 APOT 的肿瘤上皮细胞与成釉细胞蛋白、鞘蛋白、釉原蛋白和犬 APOT 淀粉样蛋白的抗体发生局部反应。犬 APOT 淀粉样蛋白的氨基酸序列与牙釉质蛋白（如成釉细胞蛋白、鞘蛋白和牙釉原蛋白）的相似性，以及这些抗原在 APOT 淀粉样蛋白和肿瘤细胞中的表达，表明犬 APOT 淀粉样蛋白源自成釉细胞分泌的釉质蛋白。
The amyloid of canine amyloid-producing odontogenic tumor (APOT) was evaluated biochemically and immunohistochemically. The N-terminal amino-acid sequence of purified amyloid protein from a canine APOT was strikingly similar to the sequence in both rat ameloblastin and porcine sheathlin. Immunohistochemically, the amyloid in APOT from 9 dogs was strongly reactive with anti-rat ameloblastin, anti-porcine sheathlin, and anti-canine APOT amyloid and weakly reactive with anti-porcine amelogenin but negative for antibodies to cytokeratins, vimentin, desmin, alpha-smooth muscle actin, amyloid A, glial fibrillary acidic protein, or S100 protein. The neoplastic epithelial cells of APOT were focally reactive with antibodies to ameloblastin, sheathlin, amelogenin, and canine APOT amyloid. The similarity in amino-acid sequence of the amyloid protein of canine APOT to that of enamel proteins, such as ameloblastin, sheathlin, and amelogenin, and the expression of these antigens in both APOT amyloid and in the neoplastic cells suggest that the amyloid of canine APOT is derived from enamel proteins secreted by ameloblasts.