CHEMOTAXIS OF POLYMORPHONUCLEAR LEUKOCYTES FROM PATIENTS WITH DIABETES MELLITUS
CHEMOTAXIS OF POLYMORPHONUCLEAR LEUKOCYTES FROM PATIENTS WITH DIABETES MELLITUS
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DOI：
10.1056/nejm197103252841201
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发表时间：
1971-01-01
影响因子：
158.5
通讯作者：
BAUM, J
中科院分区：
文献类型：
--
作者：
MOWAT, AG;BAUM, J
用一种新的体外测定外周血多形核白细胞趋化性的方法，计算了趋化指数。31例糖尿病患者的平均趋化指数显著低于对照组(P<0.0005)。趋化功能缺陷与血浆胰岛素、血糖、血二氧化碳、血尿素氮水平无关，也与任何治疗药物无关。11名正常人的白细胞与浓度为300-900毫克/100毫升的葡萄糖孵育后，各项指标均未发生改变。用浓度为100μU~10 mU/毫升的胰岛素孵育细胞，可纠正糖尿病白细胞的趋化缺陷。含有苯酚的胰岛素比不含防腐剂的胰岛素效果差。胰岛素在没有葡萄糖的情况下无效。提示糖尿病患者白细胞趋化性的这种缺陷可能是导致感染增加的原因之一。
With a new in vitro method of measuring the chemotaxis of polymorphonuclear leukocytes from peripheral blood, a chemotactic index was calculated. The mean chemotactic index in 31 patients with diabetes mellitus was significantly less (p less than 0.0005) than that in 31 matched controls. The defect in chemotaxis could not be correlated with plasma insulin, plasma glucose, serum carbon dioxide and blood urea nitrogen values, or with any therapeutic agents. Incubation of the leukocytes from 11 normals with glucose in concentrations of 300 to 900 mg per 100 ml did not alter indexes. The defect in chemotaxis of the diabetic leukocyte was corrected by incubation of the cells with insulin in concentrations of 100 μU to 10 mU per milliliter. Insulin with phenol was less effective than insulin without preservative. Insulin was ineffective in the absence of glucose. It is suggested that this defect in the chemotaxis of diabetic leukocytes could contribute to increased infections in these patients.