De novo enteric neurogenesis in post-embryonic zebrafish from Schwann cell precursors rather than resident cell types
De novo enteric neurogenesis in post-embryonic zebrafish from Schwann cell precursors rather than resident cell types
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DOI：
10.1242/dev.186619
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发表时间：
2020-07-01
期刊：
影响因子：
4.6
通讯作者：
Bronner, Marianne E.
中科院分区：
文献类型：
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作者：
El-Nachef, Wael Noor;Bronner, Marianne E.
肠道神经系统(ENS)对于正常的胃肠功能是必不可少的。虽然肠神经起源于神经脊的胚胎来源已得到很好的证实，但关于出生后肠神经发生存在相互矛盾的证据。在这里，我们通过研究胚胎后斑马鱼胚胎中新生神经发生的起源来解决这个问题。尽管在生长过程中和损伤后会增加新的神经元，但幼虫的肠道似乎缺乏常驻的神经源性前体细胞或以Sox10、plp1a、GFAP或S100为标志的经典胶质细胞。相反，用亲脂染料或可诱导的Sox10-Cre进行谱系追踪表明，胚胎后肠道神经元起源于从脊髓迁移到肠道的干神经脊来源的雪旺细胞前体细胞。此外，5-HT4受体激动剂普鲁卡必利在正常发育和损伤后增加肠道神经发生。综上所述，这些结果表明，尽管肠道中缺乏常驻祖细胞，但胚胎后肠内神经发生在发育和损伤过程中通过肠道外源性雪旺细胞前体发生，并受到5-羟色胺受体激动剂的促进。ENS中没有经典的神经胶质细胞，这进一步表明神经脊来源的肠神经胶质细胞可能是在硬骨线之后进化的。本文有一个与之相关的“论文背后的人”采访。
The enteric nervous system (ENS) is essential for normal gastrointestinal function. Although the embryonic origin of enteric neurons from the neural crest is well established, conflicting evidence exists regarding postnatal enteric neurogenesis. Here, we address this by examining the origin of de novo neurogenesis in the postembryonic zebrafish ENS. Although new neurons are added during growth and after injury, the larval intestine appears to lack resident neurogenic precursors or classical glia marked by sox10, plp1a, gfap or s100. Rather, lineage tracing with lipophilic dye or inducible Sox10-Cre suggests that post-embryonic enteric neurons arise from trunk neural crest-derived Schwann cell precursors that migrate from the spinal cord into the intestine. Furthermore, the 5-HT4 receptor agonist prucalopride increases enteric neurogenesis in normal development and after injury. Taken together, the results suggest that despite the lack of resident progenitors in the gut, post-embryonic enteric neurogenesis occurs via gut-extrinsic Schwann cell precursors during development and injury, and is promoted by serotonin receptor agonists. The absence of classical glia in the ENS further suggests that neural crest-derived enteric glia might have evolved after the teleost lineage.This article has an associated 'The people behind the papers' interview.