Cooperative Optical Trapping of Polystyrene Microparticle and Protein Forming a Submillimeter Linear Assembly of Microparticle
Cooperative Optical Trapping of Polystyrene Microparticle and Protein Forming a Submillimeter Linear Assembly of Microparticle
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DOI：
10.1021/acs.jpcc.1c05796
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发表时间：
2021-09
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通讯作者：
Po-Wei Yi;Wei-Hsiang Chiu;T. Kudo;T. Sugiyama;Roger Bresolí‐Obach;J. Hofkens;E. Chatani;R. Yasukuni;Y. Hosokawa;S. Toyouchi;H. Masuhara
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作者：
Po-Wei Yi;Wei-Hsiang Chiu;T. Kudo;T. Sugiyama;Roger Bresolí‐Obach;J. Hofkens;E. Chatani;R. Yasukuni;Y. Hosokawa;S. Toyouchi;H. Masuhara
介电粒子和金属粒子的光学捕获在空气/溶液和玻璃/溶液界面产生不同类型的“光学演化组装”。然而，所有这些结构都有一个共同点，那就是俘获激光被散射并通过组件传播，从焦点扩展到几十微米。在目前的工作中，我们从浓缩的溶菌酶D2O溶液开始，制备了单个亚毫米级的聚苯乙烯微粒的线性组装。这种组装具有由单个微粒聚集体组成的三维线性结构，没有折叠和弯曲。事实上，它是沿着溶菌酶组件制备的，溶菌酶组件也是通过光学捕获产生的。微粒子和溶菌酶的协同捕获并不是以均匀分布的方式排列的。取而代之的是，同时制备了一种独特的异常长的微粒组装和一层密集、广泛和深度膨胀的溶菌酶层。关闭捕获激光后，通过立即移动成像平面来重建它们的形态。另外，荧光成像和拉曼散射光谱也证实了溶菌酶的组装。因此，我们认为这种协作的“光学演化组装”在制备杂化材料方面具有很大的潜力，在胶体科学、蛋白质化学和软物质等不同领域都有应用。
Optical trapping of dielectric and metal particles yields different types of “optically evolving assembly” at air/solution and glass/solution interfaces. However, all these structures have in common that the trapping laser is scattered and propagated through the assembly, expanding from the focus up to a few tens of micrometers. In the present work, we fabricate a single submillimeter linear assembly of polystyrene microparticles starting from the surface of a concentrated lysozyme D2O solution. Such assembly has a three-dimensional linear structure composed of a single microparticle aggregate without folding and bending. Indeed, it is prepared along the lysozyme assembly, which is also generated by optical trapping. The cooperative trapping of the microparticle and lysozyme did not arrange as a homogeneously distributed assembly. Instead, a unique anomalously long assembly of microparticles and a densely, widely, and deeply expanded lysozyme layer were simultaneously prepared. Their morphology was reconstructed by shifting the imaging plane immediately after switching off the trapping laser. Independently, the lysozyme assembly was also confirmed by fluorescence imaging and Raman scattering spectroscopy. Thus, we consider that the described cooperative “optically evolved assembling” has a large potential to fabricate hybrid materials with applications in different fields such as colloid science, protein chemistry, and soft matter.