Thermal conditioning of fifth‐instar Cydia pomonella (Lepidoptera: Tortricidae) affects HSP70 accumulation and insect mortality
Thermal conditioning of fifth‐instar Cydia pomonella (Lepidoptera: Tortricidae) affects HSP70 accumulation and insect mortality
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DOI：
10.1111/j.1365-3032.2006.00512.x
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发表时间：
2006-09
影响因子：
1.5
通讯作者：
X. Yin;S. Wang;J. Tang;J. Hansen;S. Lurie
中科院分区：
文献类型：
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作者：
X. Yin;S. Wang;J. Tang;J. Hansen;S. Lurie
苹果小蛾[Cydia pomonella(L.)(鳞翅目：卷蛾科)]在35℃条件下处理不同时间后和在22℃条件下恢复后，用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳法分离幼虫蛋白样品。经35℃亚致死温度处理40min、2、6和18h后，幼虫提取液中出现新的蛋白质条带。用热诱导HSP70抗体进行免疫检测表明，35°C作用2、6和18小时后的反应比35°C作用40分钟或对照组更强，在22°C的恢复期，热休克蛋白水平下降。在35摄氏度下对五龄食心蛾进行条件调节也会诱导昆虫的耐热性，并需要在致命温度下保持更长的时间以确保死亡。耐热性与热诱导HSP70蛋白的积累有关。
Abstract Levels of HSP70 protein of fifth‐instar codling moth [Cydia pomonella (L.) (Lepidoptera: Tortricidae)] are determined after conditioning at 35 °C for different times and also after recovery at 22 °C. Protein samples from larvae conditioned for different times are separated by sodium dodecyl sulphate‐polyacrylamide gel electrophoresis electrophoresis. Sub‐lethal thermal conditioning at 35 °C for 40 min, 2, 6 and 18 h induces new protein bands in the extracts from treated codling moth larvae. Immunodetection with an antibody to a heat‐inducible HSP70 indicates a stronger reaction after 35 °C for 2, 6 and 18 h than after 35 °C for 40 min or control and, during the recovery period at 22 °C, the level of heat shock protein decreases. Conditioning of fifth‐instar codling moths at 35 °C also induces thermotolerance in the insects and necessitates longer times at a lethal temperature to ensure mortality. Thermotolerance is correlated with the accumulation of heat inducible HSP70 protein.