EFFECT OF CA-2+ ON THE CILIARY BEAT FREQUENCY OF SKINNED DOG TRACHEAL EPITHELIUM
EFFECT OF CA-2+ ON THE CILIARY BEAT FREQUENCY OF SKINNED DOG TRACHEAL EPITHELIUM
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DOI：
10.1016/0034-5687(85)90036-2
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发表时间：
1985-01-01
期刊：
影响因子：
--
通讯作者：
TAKISHIMA, T
中科院分区：
文献类型：
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作者：
KAKUTA, Y;KANNO, T;TAKISHIMA, T
Beat frequency of dog tracheal ciliated epithelium was measured using a profile projector and a photomultiplier. The preparation, treated with 50 .mu.g/ml of saponin for 15 min, lost osmotic behavior and the ciliary beat depended on externally applied MgATP2- indicating that ciliated epithelium was skinned with saponin. Beat frequency of skinned cilia did not increase with Ca2+ in 0.1 mM ATP2- with no ATP-regenerating system. Under 4 mM ATP2- the beat frequency increased with an increase in Ca2+ from 0.3-10 .mu.M, although a marked beat continued in the virtual absence of Ca2+. Both Ca2+ sensitivity and maximum beat frequency increased with the addition of 2.4 .mu.M calmodulin. The effect of calmodulin inhibitor (W-7) on skinned preparations was somewhat weaker than that on intact ones. Ca2, within the physiological range of concentrations, directly activated the ciliary proteins and increased the ciliary beat frequency. The addition of calmodulin augments the effect of Ca2+ but the basal beat frequency is not Ca2+ dependent.
