Bacteriophage-Derived Peptidase CHAP(K) Eliminates and Prevents Staphylococcal Biofilms.
Bacteriophage-Derived Peptidase CHAP(K) Eliminates and Prevents Staphylococcal Biofilms.
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DOI：
10.1155/2013/625341
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发表时间：
2013
影响因子：
3.4
通讯作者：
Coffey A
中科院分区：
文献类型：
--
作者：
Fenton M;Keary R;McAuliffe O;Ross RP;O'Mahony J;Coffey A
迫切需要新的抗菌剂来消除对传统抗菌剂高度耐药的生物膜形成细菌。这种细菌的扩散可能导致农业食品部门的重大经济损失。本研究证明了噬菌体衍生的肽酶CHAPK作为快速破坏生物膜形成葡萄球菌（通常与牛乳腺炎相关）的杀生物剂的潜力。将纯化的CHAPK应用于金黄色葡萄球菌DPC 5246的生物膜，在4h内完全消除金黄色葡萄球菌的生物膜。此外，CHAPK能够防止该菌株形成生物膜。CHAPK溶素也能减少S.金黄色葡萄球菌在皮肤去殖民化模型。我们的数据表明CHAPK作为生物杀灭剂用于预防和治疗生物膜相关葡萄球菌感染或作为食品和医疗保健部门的去污剂的潜力。
New antibacterial agents are urgently needed for the elimination of biofilm-forming bacteria that are highly resistant to traditional antimicrobial agents. Proliferation of such bacteria can lead to significant economic losses in the agri-food sector. This study demonstrates the potential of the bacteriophage-derived peptidase, CHAPK, as a biocidal agent for the rapid disruption of biofilm-forming staphylococci, commonly associated with bovine mastitis. Purified CHAPK applied to biofilms of Staphylococcus aureus DPC5246 completely eliminated the staphylococcal biofilms within 4 h. In addition, CHAPK was able to prevent biofilm formation by this strain. The CHAPK lysin also reduced S. aureus in a skin decolonization model. Our data demonstrates the potential of CHAPK as a biocidal agent for prevention and treatment of biofilm-associated staphylococcal infections or as a decontaminating agent in the food and healthcare sectors.