TRICINE SODIUM DODECYL-SULFATE POLYACRYLAMIDE-GEL ELECTROPHORESIS FOR THE SEPARATION OF PROTEINS IN THE RANGE FROM 1-KDA TO 100-KDA
TRICINE SODIUM DODECYL-SULFATE POLYACRYLAMIDE-GEL ELECTROPHORESIS FOR THE SEPARATION OF PROTEINS IN THE RANGE FROM 1-KDA TO 100-KDA
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DOI：
10.1016/0003-2697(87)90587-2
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发表时间：
1987-11-01
影响因子：
2.9
通讯作者：
VONJAGOW, G
中科院分区：
文献类型：
--
作者：
SCHAGGER, H;VONJAGOW, G
描述了一种不连续的十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）系统，用于1至100 kDa范围内的蛋白质分离。与甘氨酸- sds - page系统相比，用作尾随离子的三辛可以在丙烯酰胺浓度较低的情况下对小蛋白质进行分解。在不需要使用尿素的情况下，实现了蛋白质的优异分辨率，特别是在5至20 kDa的范围内。30 kDa以上的蛋白质已经在样品凝胶中分离。因此，保证了蛋白质从样品到分离凝胶的平滑通道，并减少了过载效应。当大量的蛋白质被装载到制备凝胶中时，这是特别重要的。甘氨酸和尿素的省略防止了在随后的氨基酸测序过程中可能发生的干扰。
A discontinous sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) system for the separation of proteins in the range from 1 to 100 kDa is discribed. Tricine, used as the trailing ion, allows a resolution of small proteins as lower acrylamide concentrations than in glycine-SDS-PAGE systems. A superior resolution of proteins, especially in the range between 5 and 20 kDa, is achieved without the necessity to use urea. Proteins above 30 kDa are already destacked within the sample gel. Thus a smooth passage of the proteins from sample to separating gel is warranted and overloading effects are reduced. This is of special importance when large amounts of protein are to be loaded onto preparative gels. The omission of glycine and urea prevents disturbances which might occur in the course of subsequent amino acid sequencing.