Unraveling the Evolution of Auxin Signaling
Unraveling the Evolution of Auxin Signaling
复制标题
DOI：
10.1104/pp.110.168161
复制
发表时间：
2011-01-01
期刊：
影响因子：
7.4
通讯作者：
Beeckman, Tom
中科院分区：
文献类型：
--
作者：
De Smet, Ive;Voss, Ute;Beeckman, Tom
生长素信号是植物生长发育的核心，但对其进化起源知之甚少。虽然生长素信号传导中的关键参与者的存在已经在各种陆地植物物种中进行了分析，但在绿色藻类谱系中缺乏类似的分析。在这里，我们调查的关键球员在生长素生物学在现有的基因组绿藻物种。我们发现，生长素生物合成和生长素1（AUX 1）/类似AUX 1和P-糖蛋白/ATP结合盒亚家族B依赖性转运的遗传潜力已经存在于几个单细胞和菌落形成绿藻门物种中。此外，我们对来自圆形鞘毛藻和草地水绵的表达序列标签文库的分析，这两种绿色藻类属于链藻门分支，在进化上比绿藻门分支更接近陆地植物，揭示了部分生长素应答因子和/或生长素/吲哚-3-乙酸蛋白（生长素信号传导中的关键因子）和PIN形成样蛋白（最佳表征的生长素流出载体）的存在。虽然这些可能的生长素反应因子和生长素/吲哚-3-乙酸前体和推定的PIN形成的直系同源物的鉴定要求在测序更多的中间基因组后对其进化进行更深入的研究，但它强调了典型的生长素反应机制和生长素运输机制至少部分地在植物“迁移”到陆地栖息地之前就已经存在。
Auxin signaling is central to plant growth and development, yet hardly anything is known about its evolutionary origin. While the presence of key players in auxin signaling has been analyzed in various land plant species, similar analyses in the green algal lineages are lacking. Here, we survey the key players in auxin biology in the available genomes of Chlorophyta species. We found that the genetic potential for auxin biosynthesis and AUXIN1 (AUX1)/LIKE AUX1- and P-GLYCOPROTEIN/ATP-BINDING CASSETTE subfamily B-dependent transport is already present in several single-celled and colony-forming Chlorophyta species. In addition, our analysis of expressed sequence tag libraries from Coleochaete orbicularis and Spirogyra pratensis, green algae of the Streptophyta clade that are evolutionarily closer to the land plants than those of the Chlorophyta clade, revealed the presence of partial AUXIN RESPONSE FACTORs and/or AUXIN/INDOLE-3-ACETIC ACID proteins (the key factors in auxin signaling) and PIN-FORMED-like proteins (the best-characterized auxin-efflux carriers). While the identification of these possible AUXIN RESPONSE FACTOR-and AUXIN/INDOLE-3-ACETIC ACID precursors and putative PIN-FORMED orthologs calls for a deeper investigation of their evolution after sequencing more intermediate genomes, it emphasizes that the canonical auxin response machinery and auxin transport mechanisms were, at least in part, already present before plants "moved" to land habitats.