Myocardial high-energy phosphates in reactive hyperemia.
Myocardial high-energy phosphates in reactive hyperemia.
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反应性充血中的心肌高能磷酸盐。
DOI：
10.1152/ajpheart.1990.259.4.h1190
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Weiner,MW
中科院分区：
文献类型：
--
作者：
Schwartz,GG;Schaefer,S;Gober,J;Meyerhoff,DJ;Smekal,A;Massie,B;Weiner,MW
本研究使用31 P核磁共振（NMR）光谱来确定心肌反应性充血（RH）的幅度和持续时间是否反映了心肌高能磷酸盐（HEP）代谢的相应紊乱，或者在RH期间冠状动脉血流量（CBF）和HEP代谢是否分离。12只开胸麻醉猪在冠状动脉前降支闭塞期间和闭塞24 s后进行了研究。用多普勒探头测量脑血流速度。通过对来自多个遮挡的相应短数据块进行求和来获得核磁共振时间分辨率（4.8秒）。在闭塞期间，磷酸肌酸（PCr）下降至对照组的65 +/- 5%（平均值+/- SE），同时Pi +磷酸单酯区域的光谱强度增加。在20 s的复流时，HEP已恢复到对照水平，但CBF仍升高至对照的282 +/- 18%，并在另外53 +/- 7 s内保持升高，在此期间发生了总RH流量的44 +/- 6%。因此，在RH期间，CBF的控制与心肌HEPs的水平并不密切相关，RH的持续时间并不反映心肌HEPs的长期消耗。
This study used 31P nuclear magnetic resonance (NMR) spectroscopy to determine whether the magnitude and duration of myocardial reactive hyperemia (RH) reflect a commensurate derangement of myocardial high-energy phosphate (HEP) metabolism, or if coronary blood flow (CBF) and HEP metabolism are dissociated during RH. Twelve open-chest anesthetized pigs were studied during and after 24 s occlusion of the anterior descending coronary artery. CBF velocity was measured with a Doppler probe. NMR time resolution (4.8 s) was obtained by summing corresponding short blocks of data from multiple occlusions. During occlusion, phosphocreatine (PCr) declined to 65 +/- 5% (mean +/- SE) of control accompanied by increased spectral intensity of the Pi + phosphomonester region. By 20 s of reflow, HEPs had returned to control levels, but CBF was still elevated at 282 +/- 18% of control and remained elevated for an additional 53 +/- 7 s, during which 44 +/- 6% of total RH flow occurred. Therefore, the control of CBF is not closely coupled to the levels of myocardial HEPs during RH and the duration of RH does not reflect prolonged depletion of myocardial HEPs.