Immunophenotypic analysis of neoplastic cells in follicular dendritic cell sarcoma.
Immunophenotypic analysis of neoplastic cells in follicular dendritic cell sarcoma.
复制标题
滤泡树突细胞肉瘤中肿瘤细胞的免疫表型分析。
作者：
G. Pallesen;O. Myhre
存在源自抗原呈递滤泡树突状细胞（FDCs）的肉瘤，但从未得到证实。本报告描述了一例39岁男性腋窝淋巴结肿瘤，其形态学、酶组织化学和免疫表型数据与FDC起源的恶性细胞一致。形态学上，肿瘤呈双核和多核的趋势，呈层状生长模式，初步诊断为恶性纤维组织细胞瘤。然而，肿瘤细胞与发育良好的桥粒相互连接。酶组织化学显示有较强的5′-核苷酸酶活性。免疫组织学分析显示，肿瘤细胞强烈表达三种fdc特异性抗原中的两种（Ki-M4、BU-10和R4/23）。几乎没有所有的髓细胞标记物。与正常FDCs一样，白细胞抗原CD35、CD19、CD21和CD23表达强烈，CD4抗原表达弱。未见CD45抗原染色，未见非造血细胞标记物表达。正常FDC的起源尚不清楚。鉴于一些证据表明FDC起源于骨髓，该细胞可能代表一个不同于其他已知骨髓干细胞衍生的细胞谱系，因为其免疫表型与它们有很大不同。
The existence of a sarcoma derived from the antigen-presenting follicular dendritic cells (FDCs) has been assumed but never confirmed. This report describes a tumor from axillary lymph nodes of a 39-year-old male in which the morphologic, enzyme histochemical, and immune phenotypic data are consistent with the malignant cells being of FDC origin. Morphologically, the tumor showed a tendency toward bi- and polynucleation with areas of storiform growth pattern, resulting in an initial diagnosis of malignant fibrous histiocytoma. However, the tumor cells had interconnections with well-developed desmosomes. Enzyme histochemistry revealed strong 5'-nucleotidase activity. Immunohistologic analysis showed that tumor cells expressed two of three FDC-specific antigens (Ki-M4, BU-10, and R4/23) strongly. Virtually all myelomonocytic markers were absent. Like normal FDCs, the leukocyte antigens CD35, CD19, CD21, and CD23 were expressed strongly and CD4 antigen weakly. No staining was evident for CD45 antigen, and no nonhemopoietic cell markers were expressed. The origin of the normal FDC is obscure. Given some evidence suggesting a bone marrow origin for the FDC, this cell may represent a lineage distinct from other known cell lineages derived from bone marrow stem cells since its immune phenotype differs considerably from them.