Simple colorimetry of glycated serum protein in a centrifugal analyzer.
Simple colorimetry of glycated serum protein in a centrifugal analyzer.
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DOI：
10.1093/clinchem/30.10.1686
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发表时间：
1984-10
影响因子：
9.3
通讯作者：
D. Lloyd;J. Marples
中科院分区：
文献类型：
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作者：
D. Lloyd;J. Marples
最近描述的（Clin Chim Acta 127：87-95，1982）血清中糖化蛋白的比色测定法利用了它们在碱性溶液中还原3，3 ′-（3，3 ′-二甲氧基-4，4 ′-亚联苯基）双[2-（对硝基苯基）-5-苯基-2H-四唑鎓氯化物]（硝基蓝四唑鎓）的酮胺活性;作者将其称为“果糖胺测定法”。“该方法简单，只需要添加一种试剂并测量5分钟内的吸光度变化;结果相对于合成标准品表示。我们已经调整了这在离心分析仪中使用，并报告其性能，无论是分析和高血糖症的指标。精密度良好（批间CV 2.1%），试剂稳定且廉价，程序快速（每小时75个样品）。白蛋白影响测量，但对于浓度大于或等于35 g/L，这不是严重问题。正常人群和糖尿病人群可以明显区分（p <0.001）。该测试检测了30名未经治疗的糖尿病患者中的25名（84%），并给出了4个假阳性（8%）。结果与空腹血糖（r = 0.87）和血红蛋白A1（r = 0.80）相关良好。
A recently described (Clin Chim Acta 127: 87-95, 1982) colorimetric assay for glycated proteins in serum exploits their ketoamine activity to reduce 3,3'-(3,3'-dimethoxy-4,4'-biphenylylene)bis [2-(p-nitrophenyl)-5-phenyl-2H-tetrazolium chloride] (nitroblue tetrazolium) in alkaline solution; the authors termed this the "fructosamine assay." The method is simple, requiring only the addition of one reagent and measurement of the absorbance change during 5 min; results are expressed relative to a synthetic standard. We have adapted this for use in a centrifugal analyzer and report its performance, both analytically and as an index of hyperglycemia. Precision is good (between-batch CV 2.1%), the reagent is stable and inexpensive, and the procedure is rapid (75 samples per hour). Albumin influences the measurement, but for concentrations greater than or equal to 35 g/L this was not a serious problem. Normal and diabetic populations can be clearly discriminated (p less than 0.001). The test detected 25 (84%) of the 30 untreated diabetics studied and gave four false positives (8%). The results correlate well with those for glucose in plasma of fasting subjects (r = 0.87) and for hemoglobin A1 (r = 0.80).