Disruption of hex1 in Trichoderma atroviride leads to loss of Woronin body and decreased tolerance to dichlorvos
Disruption of hex1 in Trichoderma atroviride leads to loss of Woronin body and decreased tolerance to dichlorvos
复制标题
深绿色木霉中 hex1 的破坏导致 Woronin 体丧失并降低对敌敌畏的耐受性
DOI：
10.1007/s10529-013-1409-x
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发表时间：
2014-04
期刊：
影响因子：
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通讯作者：
Jie Chen
中科院分区：
文献类型：
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作者：
Qiong Wu;ChuanJin Yu;WanJun Shi;Jie Chen
木霉菌对有机磷农药的耐受性是其在农药污染环境生物修复中应用的必要条件。在某些情况下，这种要求也是这些真菌与化学杀虫剂协同使用的关键，目的是扩大控制目标的范围，以包括植物病原体和虫害。然而，木霉菌对有机磷农药的耐药机制尚不清楚。为了解决这个问题，我们通过敲除它来分析推测的敌敌畏耐受基因Hex1的功能。该突变株的虫体缺失，对有机磷农药敌敌畏的耐受性降低。此外，HEX1定位于菌丝体的隔膜上，可能参与控制敌敌畏在细胞内的运动。因此，Hex1与木霉对敌敌畏的耐受性和虫体的形成有关。
The tolerance of Trichoderma species to organophosphorus pesticides is necessary for their application in the bioremediation of pesticide-polluted environments. In some cases, such a requirement is also key to the synergistic use of these fungi with chemical pesticides, aiming to broaden the scope of control targets to include both plant pathogens and insect pests. However, the mechanism of Trichoderma tolerance of organophosphorus pesticides remains unclear. To address this, we have analyzed the function of the putative dichlorvos-tolerance gene hex1 by knocking it out. The hex1-deleted mutant showed loss of Woronin bodies and decreased tolerance to the organophosphate, dichlorvos. Moreover, HEX1 localizes at the septal plugs in mycelium which may be involved in controlling intracellular movement of dichlorvos. hex1 thus is involved the tolerance to dichlorvos and the formation of Woronin bodies in Trichoderma atroviride.