Intermittent cold exposure improves glucose homeostasis associated with brown and white adipose tissues in mice.
Intermittent cold exposure improves glucose homeostasis associated with brown and white adipose tissues in mice.
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DOI：
10.1016/j.lfs.2015.07.030
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发表时间：
2015-10-15
期刊：
影响因子：
6.1
通讯作者：
Sun Q
中科院分区：
文献类型：
--
作者：
Wang TY;Liu C;Wang A;Sun Q
不同类型脂肪的发现与肥胖相关代谢紊乱的发病机制有关。然而，早期和间歇性暴露于低温对成年期全身代谢变化的影响仍不清楚。
为了阐明低温暴露对脂肪组织代谢功能的影响，我们研究了小鼠在间歇性寒冷暴露下的葡萄糖稳态、棕色脂肪组织（BAT）的激活以及白色脂肪组织（WAT）的“褐变”情况。小鼠每天暴露于4℃环境2小时，每周5天，持续14周。通过腹腔内葡萄糖耐量试验和胰岛素耐量试验检测葡萄糖稳态；利用体内磁共振成像评估体脂肪量；主要通过正电子发射断层扫描/计算机断层扫描检测棕色脂肪组织活性；并使用免疫组织化学方法评估白色脂肪组织的“褐变”。
我们的研究结果表明，14周的寒冷暴露可改善葡萄糖耐量，增强胰岛素敏感性，降低附睾和腹膜后白色脂肪组织的相对重量，增加皮下脂肪组织中解偶联蛋白1（UCP1）和过氧化物酶体增殖物激活受体γ共激活因子1α（PGC1α）的表达。
生命早期间歇性暴露于低温可能会改善全身葡萄糖稳态并诱导白色脂肪组织“褐变”，这表明环境低温暴露可能是一种对代谢紊乱有前景的干预措施。
The discovery of different shades of fat has been implicated in the pathogenesis of obesity-related metabolic disorders. However, the effects of early and intermittent exposure to cold temperature on systemic metabolic changes in adult life remain unclear. To elucidate the impact of cold temperature exposure on metabolic function of adipose tissues, we investigated the glucose homeostasis, activation of brown adipose tissue (BAT) and “browning” of white adipose tissue (WAT) in mice in response to intermittent cold exposure. Mice were exposed to 4 °C, 2 hours per day and 5 days per week, for 14 weeks. Glucose homeostasis was tested via intraperitoneal glucose tolerance test and insulin tolerance test; body fat mass was evaluated using in vivo magnetic resonance imaging; BAT activity was detected primarily by positron emission tomography/computed tomography; and WAT “browning” was evaluated using immunohistochemistry. Our results showed that 14-week cold exposure improved glucose tolerance and enhanced insulin sensitivity, reduced the relative weights of epididymal and retroperitoneal WAT, increased expressions of UCP1 and PGC1α in subcutaneous adipose tissue. Intermittent exposure to cold temperature in early life may improve systemic glucose homeostasis and induce WAT “browning”, suggesting that ambient cold temperature exposure may serve as a promising intervention to metabolic disorders.