Allelic nomenclature for the duplicated MHC class II DQ genes in sheep.
Allelic nomenclature for the duplicated MHC class II DQ genes in sheep.
复制标题
绵羊中重复的 MHC II 类 DQ 基因的等位基因命名法。
DOI：
10.1007/s00251-018-1096-9
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发表时间：
2019
期刊：
影响因子：
3.2
通讯作者：
Ballingall KT
中科院分区：
文献类型：
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作者：
Ballingall KT
绵羊CD 4 ~+T细胞基因上的主要MHC Ⅱ类分子来自DR和DQ基因。奥维德的DRB 1和它的伙伴DRA位点的等位基因命名系统是可用的;然而，DQ基因没有正式的命名，这在研究界造成了歧义。绵羊MHC单倍型至少包括两对DQA和DQB基因，命名为DQA 1，DQB 1和DQA 2，DQB 2，这两组基因都是多态的，并且似乎都是功能性的。在许多单倍型中，DQA 1基因座似乎缺失（DQA 1-null），并被第二个基因座DQA 2-like取代。在这里，我们根据序列相似性和系统发育聚类来确定等位基因家族，这些等位基因对应于在以前的纯合动物基因组和转录本分析中确定的每个DQA和DQB基因。利用这些标准对序列进行聚类，我们命名了82个来自绵羊（Ovis aries）的全长和部分cDNA转录本，它们对应于Ovar-DQA 1、DQA 2、DQA 2-like、DQB 1、DQB 2和DQB 2-like基因的等位基因，并通过IPD-MHC数据库为研究界提供了相关的序列资源。这为绵羊MHC中DQ基因的命名和注释奠定了基础，并可用作其他反刍动物物种中DQ基因的模板，最终将支持家畜传染病的研究。
The principal MHC class II molecules involved in the presentation of peptides to the antigen specific receptors on CD4+T cells genes in sheep are derived fromDRandDQgenes. Allelic nomenclature systems for theDRB1and its partnerDRAloci are available for Ovid’s; however, no official nomenclature is available for theDQgenes which creates ambiguity within the research community. Ovine MHC haplotypes include at least two pairs ofDQAandDQBgenes, termedDQA1,DQB1andDQA2,DQB2and both sets are polymorphic and both seem to be functional. In a number of haplotypes, theDQA1locus appears to be absent (DQA1-null) and is replaced by a second locus termedDQA2-like. Here, we identify families of alleles based on sequence similarity and phylogenetic clustering which correspond to each of theDQAandDQBgenes identified in previous genomic and transcript analyses of homozygous animals. Using such criteria to cluster sequences, we have named 82 full-length and partial cDNA transcripts derived from domestic sheep (Ovis aries) which correspond to alleles at theOvar-DQA1,DQA2,DQA2-like,DQB1,DQB2andDQB2-likegenes and provide associated sequence resources available to the research community through the IPD-MHC Database. This sets the basis for naming and annotation ofDQgenes within the ovine MHC and may be used as a template forDQgenes in other ruminant species which will ultimately support research in livestock infectious disease.