A dominant-negative ESCRT-III protein perturbs cytokinesis and trafficking to lysosomes
A dominant-negative ESCRT-III protein perturbs cytokinesis and trafficking to lysosomes
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DOI：
10.1042/bj20071296
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发表时间：
2008-04-15
影响因子：
4.1
通讯作者：
Whitley, Paul
中科院分区：
文献类型：
--
作者：
Dukes, Joseph D.;Richardson, Judith D.;Whitley, Paul
在真核细胞中，胞质分裂的完成依赖于膜运输事件以将膜递送到分裂位点。高尔基体和回收胞内体衍生的蛋白质是胞质分裂的终末阶段所必需的。最近，ESCRT（转运所需的内体分选复合物）的蛋白质亚基，通常参与晚期内体到溶酶体的运输，也参与了凋亡。在这里，我们报告说，亚基，CHMP 3（带电多泡体蛋白-3），ESCRT-III定位在中间体。CHMP 3的C-末端自身抑制结构域的缺失抑制胞质分裂。在中间体，CHMP 3不与Rab 11共定位，表明它不存在于再循环内体上。这些结果结合起来提供了令人信服的证据，参与后期内体功能的蛋白质是胞质分裂末期所必需的。
In eukaryotic cells, the completion of cytokinesis is dependent on membrane trafficking events to deliver membrane to the site of abscission. Golgi and recycling endosomal-derived proteins are required for the terminal stages of cytokinesis. Recently, protein subunits of the ESCRT (endosomal sorting complexes required for transport) that are normally involved in late endosome to lysosome trafficking have also been implicated in abscission. Here, we report that a subunit, CHMP3 (charged multivesicular body protein-3), of ESCRT-III localizes at the midbody. Deletion of the C-terminal autoinhibitory domain of CHMP3 inhibits cytokinesis. At the midbody, CHMP3 does not co-localize with Rab11, suggesting that it is not present on recycling endosomes. These results combined provide compelling evidence that proteins involved in late endosomal function are necessary for the end stages of cytokinesis.