Contribution of human α-defensin 1, 2, and 3 to the anti-HIV-1 activity of CD8 antiviral factor (Retracted Article)
Contribution of human α-defensin 1, 2, and 3 to the anti-HIV-1 activity of CD8 antiviral factor (Retracted Article)
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DOI：
10.1126/science.1076185
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发表时间：
2002-11-01
期刊：
影响因子：
56.9
通讯作者：
Ho, DD
中科院分区：
文献类型：
--
作者：
Zhang, LQ;Yu, WJ;Ho, DD
自 1986 年以来，人们就知道来自某些免疫学稳定的 HIV-1 感染者的 CD8 T 淋巴细胞会分泌可溶性因子（称为 CAF），抑制 HIV-1 复制。然而，尽管进行了广泛的搜索，CAF 的身份仍然难以捉摸。通过蛋白质芯片技术，我们鉴定了当 HIV-1 感染长期无进展者的 CD8 T 细胞受到刺激时分泌的蛋白质簇。根据特异性抗体识别和氨基酸测序，这些蛋白质被鉴定为α-防御素1、2和3。 CAF 活性被人类 α-防御素特异性抗体消除或中和。 α-防御素的合成和纯化制剂也在体外抑制 HIV-1 分离株的复制。综上所述，我们的结果表明，α-防御素 1、2 和 3 共同解释了 CAF 的大部分抗 HIV-1 活性，而这些活性并非归因于 β-趋化因子。
It has been known since 1986 that CD8 T lymphocytes from certain HIV-1- infected individuals who are immunologically stable secrete soluble factor, termed CAF, that suppresses HIV-1 replication. However, the identity of CAF remained elusive despite an extensive search. By means of protein-chip technology, we identified cluster of proteins that were secreted when CD8 T cells from long-term nonprogressors with HIV-1 infection were stimulated. These proteins were identified as alpha-defensin 1, 2, and 3 on the basis of specific antibody recognition and amino acid sequencing. CAF activity was eliminated or neutralized by an antibody specific for human alpha-defensins. Synthetic and purified preparations of alpha-defensins also inhibited the replication of HIV-1 isolates in vitro. Taken together, our results indicate that alpha-defensin 1, 2, and 3 collectively account for much of the anti HIV-1 activity of CAF that is not attributable to beta-chemokines.