ACTIVE HAGEMAN FACTOR - PLASMA LYSOKINASE OF HUMAN FIBRINOLYTIC SYSTEM
ACTIVE HAGEMAN FACTOR - PLASMA LYSOKINASE OF HUMAN FIBRINOLYTIC SYSTEM
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DOI：
10.1172/jci104590
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发表时间：
1962-01-01
影响因子：
15.9
通讯作者：
FERGUSON, JH
中科院分区：
文献类型：
--
作者：
IATRIDIS, SG;FERGUSON, JH
本文报道了一种简便、敏感的方法--纤溶酶-纤溶酶生成试验（PGT），用于研究Hageman因子在血浆纤溶酶（纤溶酶原激活剂）生成中的作用。该试验包括测定从稀释血浆（20倍，用蒸馏水）中采集的样品的优球蛋白溶解时间，调节至pH 5.3，并在37 ℃下孵育。结果表明，在正常血浆的PGT中开发的因子是纤溶酶原激酶，并且其生成取决于存在足够量的纤溶酶原激酶原（前激活剂）和Hageman因子，后者充当催化剂。在没有Hageman因子的情况下，PGT中不形成纤溶酶。还获得的证据表明，纤溶酶是无关的血浆纤溶酶的生成和相互作用的因素，形成它。
A simple and sensitive method, the plasmino-plastin generation test (PGT), has been used to investigate the role of Hageman factor in the generation of plasma plasminoplastin (plasmino-gen activator). The test involves determinations of the euglobulin lysis time of samples taken from diluted plasma (20-fold, with distilled water), adjusted to pH 5.3, and incubated at 37[degree]C. The results indicate that the factor developed in the PGT of normal plasma is plasminoplastin and that its generation depends on the presence of adequate amounts of proplasminoplastin (proactivator) and of Hageman factor, with the latter acting as catalyst. In the absence of Hageman factor, no plasminoplastin is formed in the PGT. Evidence also was obtained that plasmin is unrelated to the generation of plasma plasminoplastin and to the factors which interact to form it.