The prognostic significance of SHP2 and its binding protein Hook1 in non-small cell lung cancer
The prognostic significance of SHP2 and its binding protein Hook1 in non-small cell lung cancer
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SHP2及其结合蛋白Hook1在非小细胞肺癌中的预后意义
DOI：
10.2147/ott.s210223
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发表时间：
2019-01-01
影响因子：
4
通讯作者：
Wang, Linrun
中科院分区：
文献类型：
--
作者：
He, Lingjuan;Li, Yinyan;Wang, Linrun
背景我们以前报道过Hook 1抑制SHP 2磷酸酶活性调节肺癌上皮-间质转化（EMT）。本研究旨在全面分析SHP 2和Hook 1在非小细胞肺癌（NSCLC）中的表达及其与患者预后的关系。材料与方法121例非小细胞肺癌患者纳入本研究。用免疫组化和Western blot分析SHP 2和Hook 1的表达。采用考克斯比风险多变量分析和log-rank检验分析NSCLC患者的总生存率。结果SHP 2蛋白在肿瘤组织中的阳性表达率为58.4%。SHP 2和Hook 1的表达之间存在显著相关性。基于Western blot分析，我们发现Hook 1在NSCLC组织中下调，而SHP 2在NSCLC组织中有过表达的趋势，但与正常肺组织中的水平相比无统计学意义。低水平SHP 2表达的NSCLC患者的中位总生存期（OS）优于高水平SHP 2表达的患者（40 vs 24个月，p=0.004）。多因素分析结果显示，SHP 2的表达水平是影响OS的独立预后因素。结论SHP 2在NSCLC中可能发挥重要作用，有望成为NSCLC的临床生物标志物。
Background We previously reported that Hook1 inhibits the phosphatase activity of SHP2 in the regulation of the epithelial-mesenchymal transition (EMT) in lung cancer. In this study, we performed a comprehensive analysis of SHP2 and Hook1 expression and relationships with the prognosis of patients with non-small cell lung cancer (NSCLC). Materials and methods A total of 121 patients with NSCLC were included in this study. Expression of SHP2 and Hook1 was assessed by immunohistochemistry and Western blot analysis. The overall survival rate of NSCLC patients was analysed using Cox’s ratio hazard multivariate analysis and the log-rank test. Results In tumour tissue specimens, positive expression rates of SHP2 proteins were 58.4% by immunohistochemical analysis. A significant correlation between expression of SHP2 and that of Hook1 was observed. Based on Western blot analysis, we found that Hook1 was downregulated and that SHP2 has a tendency to overexpress without statistical significance in NSCLC tissues compared with their levels in normal lung tissues. The median overall survival (OS) of NSCLC patients who presented low levels of SHP2 expression were better (40 vs 24 months, p=0.004) than those of patients who exhibited high levels of SHP2 expression. The results of multivariate analysis showed that the level of SHP2 expression was an independent prognostic factor for OS. Conclusion SHP2 might play an important role in NSCLC and has the potential to serve as a clinical biomarker or NSCLC.