Evolution of γ-butyrolactone synthases and receptors in Streptomyces
Evolution of γ-butyrolactone synthases and receptors in Streptomyces
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DOI：
10.1111/j.1462-2920.2007.01314.x
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发表时间：
2007-08-01
影响因子：
5.1
通讯作者：
Horinouchi, Sueharu
中科院分区：
文献类型：
--
作者：
Nishida, Hiromi;Ohnishi, Yasuo;Horinouchi, Sueharu
γ-丁内酯调节系统触发革兰氏阳性、土栖、丝状细菌属链霉菌的次生代谢和/或形态发育。 γ-丁内酯的代表是灰色链霉菌中的A因子。 AfsA 是 A 因子生物合成的酶，ArpA 是 A 因子受体蛋白，以 A 因子为配体充当转录因子。对链霉菌中广泛分布的AfsA及其同源物和ArpA及其同源物之间的进化关系进行分析，发现它们的系统发育树拓扑存在巨大差异。因此，给定链霉菌菌株中 AfsA 同源物和 ArpA 同源物的组合似乎在进化过程中发生了变化。即使afsA同源物和arpA同源物位于染色体上相邻，在一些链霉菌菌株中个体的进化历史也被发现是不同的。此外，系统发育分析表明，在细菌进化过程中γ-丁内酯受体蛋白出现之前，祖先ArpA蛋白已经存在并作为DNA结合蛋白而不是γ-丁内酯受体发挥作用。一些链霉菌菌株具有编码AfsA/ArpA同源物的质粒，这表明质粒在afsA/arpA同源物的分布中发挥了重要作用。在进化过程中，一旦链霉菌菌株获得了不同的 afsA/arpA 同源物，它可能会开发出一种新的 γ-丁内酯调节系统，从而重建次级代谢和/或形态发生的调节系统。这个想法与链霉菌菌株中 AfsA 同源物和 ArpA 同源物的不同组合是一致的。
The gamma-butyrolactone regulatory system triggers secondary metabolism and/or morphological development in the Gram-positive, soil-dwelling, filamentous bacterial genus Streptomyces. The representative of the gamma-butyrolactones is A-factor in Streptomyces griseus. AfsA is an enzyme for A-factor biosynthesis and ArpA is the A-factor receptor protein that serves as a transcriptional factor by using A-factor as the ligand. Analysis of evolutional relations between AfsA including its homologues and ArpA including its homologues, all of which are widely distributed in Streptomyces, revealed great differences in the topologies of their phylogenetic trees. Therefore, the combinations of AfsA homologues and ArpA homologues in a given Streptomyces strain appear to have changed during the evolution. Even if an afsA homologue and an arpA homologue locate adjacently on the chromosome, the evolutional history of the individuals was found to be different in some Streptomyces strains. In addition, the phylogenetic analyses suggested that the ancestral ArpA protein had existed and functioned as a DNA-binding protein, not as a gamma-butyrolactone receptor, before the appearance of a gamma-butyrolactone receptor protein in the course of the bacterial evolution. Some Streptomyces strains have plasmids encoding AfsA/ArpA homologues, which suggests that plasmids have played an important role in the distribution of afsA/arpA homologues. During the evolution, once a Streptomyces strain acquired different afsA/arpA homologues, it may have developed a new y-butyrolactone regulatory system reconstructing the regulatory systems for secondary metabolism and/or morphogenesis. This idea is consistent with the diverged combination of AfsA homologues and ArpA homologues in a Streptomyces strain.