Characteristics of Nipah virus and Hendra virus replication in different cell lines and their suitability for antiviral screening
Characteristics of Nipah virus and Hendra virus replication in different cell lines and their suitability for antiviral screening
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DOI：
10.1016/j.virusres.2009.01.014
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发表时间：
2009-06-01
期刊：
影响因子：
5
通讯作者：
Mungall, Bruce A.
中科院分区：
文献类型：
--
作者：
Aljofan, Mohamad;Saubern, Simon;Mungall, Bruce A.
我们最近描述了一种适用于亨尼帕病毒抗病毒鉴定的高通量筛选试验的开发和验证。虽然我们确信该试验是稳健和有效的，但我们希望研究一系列替代细胞系中的试验性能，以确定是否可以提高试验灵敏度和特异性。我们评估了10种不同的细胞系对亨德拉和尼帕病毒感染的敏感性，以及它们检测广谱抗病毒药物利巴韦林和9种使用我们的初步筛选方法鉴定的新型抗病毒药物的作用的灵敏度。就病毒复制而言，将细胞系分为三类。病毒在Vero和BSR细胞中增殖最好，其次是Hep-2、HeLa、BHK-21和M17细胞。在BAEC、MMEC、A549和ECV 304细胞中观察到最低水平的RNA复制和病毒蛋白表达。八种细胞系似乎在区分三氮唑核苷的抗病毒作用方面具有类似的效果（
We have recently described the development and validation of a high throughput screening assay suitable for henipavirus antiviral identification. While we are confident this assay is robust and effective, we wished to investigate assay performance in a range of alternative cell lines to determine if assay sensitivity and specificity could be improved. We evaluated ten different cell lines for their susceptibility to Hendra and Nipah virus infection and their sensitivity of detection of the effects of the broad spectrum antiviral, ribavirin and nine novel antivirals identified using our initial screening approach. Cell lines were grouped into three categories with respect to viral replication. Virus replicated best in Vero and BSR cells, followed by Hep-2, HeLa, BHK-21 and M17 cells. The lowest levels of RNA replication and viral protein expression were observed in BAEC, MMEC, A549 and ECV304 cells. Eight cell lines appeared to be similarly effective at discriminating the antiviral effects of ribavirin (