Electrical conductivity, transfer of hydrogen ions in lipid bilayer membranes and uncoupling effect induced by pentachlorobenzenethiol (pentachlorothiophenol).
Electrical conductivity, transfer of hydrogen ions in lipid bilayer membranes and uncoupling effect induced by pentachlorobenzenethiol (pentachlorothiophenol).
复制标题
电导率、氢离子在脂质双层膜中的转移以及五氯苯硫酚（五氯苯硫酚）诱导的解偶联效应。
DOI：
10.1007/bf01870365
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Hsu,K
中科院分区：
文献类型：
--
作者：
Smejtek,P;Jayaweera,AR;Hsu,K
Pentachlorobenzenethiol (PCBT) has been considered an anomalous uncoupler. It was reported as active in mitochondria, but not effective in inducing electrical conductivity in lipid bilayer membranes. We have overcome the experimental difficulties associated with accurate determination of the induced conductivity. The main contributing factors to the difficulties, we discovered, are the photolability and the low solubility of the compound in aqueous medium. We have conclusively demonstrated that PCBT does induce conductivity in lipid bilayers and compared this conductance with its uncoupling activity reported by other investigators in the literature. We present the results of steady-state current-voltage measurements: conductance dependence on applied voltage for various values of pH, buffer strength and PCBT concentration, as well as the dependence of the conductance on pH, buffer strength and PCBT concentration in the limit of zero applied voltage. We have also compared the above results with those obtained previously with pentachlorophenol. Our experimental results on PCBT-induced membrane conductance suggest that PCBT belongs to class II uncouplers and that “disulfide dimer” of PCBT is membrane inactive. Thus the replacement of oxygen in molecular structure of pentachlorophenol (R-OH) by sulfur (R-SH) does not change the protonophoretic activity of the compound. The conductivity of a membrane is due to PCBT-induced hydrogen ion transfer and it was found to be limited by the kinetics of reactions coupled to transmembrane charge transfer. The kinetic limitations became prominent at higher PCBT concentrations and at both low and high pH. Our findings support the existence of correlation between the uncoupling effect and the magnitude of membrane electrical conductance associated with the protonophoretic effect because (1) the pH dependence of PCBT-induced membrane conductance was found to be similar to the pH dependence of its uncoupling activity in rat liver mitochondria, and (2) PCBT, which induced greater membrane conductivity than PCP, was also found to be a more effective uncoupler.
