Murine macrophage interleukin-1 release by capsularlike serotype-specific polysaccharide antigens of Actinobacillus actinomycetemcomitans
Murine macrophage interleukin-1 release by capsularlike serotype-specific polysaccharide antigens of Actinobacillus actinomycetemcomitans
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放线放线杆菌荚膜样血清型特异性多糖抗原释放小鼠巨噬细胞白介素-1
DOI：
10.1128/iai.59.1.18-23.1991
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发表时间：
1991
影响因子：
3.1
通讯作者：
T. Koga
中科院分区：
文献类型：
--
作者：
T. Takahashi;T. Nishihara;Y. Ishihara;K. Amano;N. Shibuya;I. Moro;T. Koga
用高压灭菌法从伴放线放线杆菌ATCC 29523（血清型a）、Y 4（血清型B）和NCTC 9710（血清型c）的全细胞中提取血清型特异性多糖抗原（SPAs），并通过DEAE-Sephadex A-25和Sephacryl S-300柱层析进行纯化。Y 4 SPA诱导P388 D1小鼠巨噬细胞释放白细胞介素-1（IL-1）。多粘菌素B对IL-1的释放几乎没有影响。兔抗鼠IL-1血清能强烈抑制Y 4 SPA刺激的P388 D1细胞培养上清和亚促分裂剂量的刀豆蛋白A诱导的C3 H/HeJ小鼠胸腺细胞增殖。在Sephacryl S-200上对Y 4 SPA刺激的巨噬细胞的培养上清液进行凝胶过滤，结果显示洗脱出在对应于约16.5kDa的点处的IL-1峰。菌株ATCC 29523和NCTC 9710的SPA诱导IL-1释放的能力低于Y 4 SPA。血清型A和C抗原的IL-1释放能力通过两种多糖的脱乙酰化而增强，这表明这些抗原的乙酰基可能阻碍抗原与巨噬细胞之间的相互作用。
Serotype-specific polysaccharide antigens (SPAs) were extracted from whole cells of Actinobacillus actinomycetemcomitans ATCC 29523 (serotype a), Y4 (serotype b), and NCTC 9710 (serotype c) by autoclaving and purified by chromatography on DEAE-Sephadex A-25 and Sephacryl S-300 columns. Y4 SPA induced interleukin-1 (IL-1) release by P388D1 murine macrophages. Polymyxin B had virtually no effect on the release of IL-1. Rabbit anti-murine IL-1 serum strongly suppressed the proliferation of C3H/HeJ mouse thymocytes induced with the culture supernatants of Y4 SPA-stimulated P388D1 cells and a submitogenic dose of concanavalin A. Gel filtration of the culture supernatants of Y4 SPA-stimulated macrophages on Sephacryl S-200 showed that an IL-1 peak at a point corresponding to approximately 16.5 kDa was eluted. The ability of SPAs from strains ATCC 29523 and NCTC 9710 to induce the release of IL-1 was lower than that of Y4 SPA. The IL-1-releasing ability of serotype a and c antigens was enhanced by deacetylation of both polysaccharides, suggesting that acetyl groups of these antigens might hinder the interaction between the antigens and macrophages.
伴放线放线杆菌菌株 Y4 脂多糖对小鼠腹腔巨噬细胞的体外影响。
影响因子：
2.8
作者：
Barker,KA;Holt,SC
通讯作者：
Holt,SC