Functional annotation of the T-cell immunoglobulin mucin family in birds.
Functional annotation of the T-cell immunoglobulin mucin family in birds.
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DOI：
10.1111/imm.12607
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发表时间：
2016-07
期刊：
影响因子：
6.4
通讯作者：
Kaiser P
中科院分区：
文献类型：
--
作者：
Hu T;Wu Z;Vervelde L;Rothwell L;Hume DA;Kaiser P
T细胞免疫球蛋白和粘蛋白（TIM）家族分子是细胞膜蛋白，优先在各种免疫细胞上表达，并参与凋亡细胞的识别和清除。除了人类和小鼠，对它们的功能知之甚少，在哺乳动物之外也一无所知。我们只确定了两个TIM基因（chTIM）在鸡基因组中，推定的直系同源哺乳动物TIM 1和TIM 4，并克隆了各自的cDNA。与哺乳动物TIM 1一样，chTIM 1表达仅限于淋巴组织和免疫细胞。基因chTIM 4编码至少五种剪接变体，其具有不同的表达谱，这些表达谱在鸡的品系之间也不同。在髓样抗原呈递细胞和γδ T细胞中检测到chTIM 4的表达，而哺乳动物TIM 4在T细胞中不表达。与哺乳动物蛋白一样，chTIM 1和chTIM 4融合蛋白与磷脂酰丝氨酸结合，从而参与凋亡细胞的识别。chTIM 4-免疫球蛋白融合蛋白对鸡T细胞也具有共刺激活性，表明在抗原呈递中的功能。
T‐cell immunoglobulin and mucin (TIM) family molecules are cell membrane proteins, preferentially expressed on various immune cells and implicated in recognition and clearance of apoptotic cells. Little is known of their function outside human and mouse, and nothing outside mammals. We identified only two TIM genes (chTIM) in the chicken genome, putative orthologues of mammalian TIM1 and TIM4, and cloned the respective cDNAs. Like mammalian TIM1, chTIM1 expression was restricted to lymphoid tissues and immune cells. The gene chTIM4 encodes at least five splice variants with distinct expression profiles that also varied between strains of chicken. Expression of chTIM4 was detected in myeloid antigen‐presenting cells, and in γδ T cells, whereas mammalian TIM4 is not expressed in T cells. Like the mammalian proteins, chTIM1 and chTIM4 fusion proteins bind to phosphatidylserine, and are thereby implicated in recognition of apoptotic cells. The chTIM4–immunoglobulin fusion protein also had co‐stimulatory activity on chicken T cells, suggesting a function in antigen presentation.