Electrochemical detection of 9-hydroxyfluorene based on the direct interaction with hairpin DNA
Electrochemical detection of 9-hydroxyfluorene based on the direct interaction with hairpin DNA
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基于与发夹 DNA 直接相互作用的 9-羟基芴的电化学检测
DOI：
10.1039/c2an36255d
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发表时间：
2013-01-01
期刊：
影响因子：
4.2
通讯作者：
Liu, Xinhui
中科院分区：
文献类型：
--
作者：
Liang, Gang;Li, Xiaohong;Liu, Xinhui
利用电化学阻抗谱、紫外-可见光谱和核磁共振氢谱研究了发夹DNA与9-羟基芴（9-OHFLU）的直接氢键作用。在此基础上，构建了一种电化学发夹型DNA传感器，用于9-OHFLU的电化学检测。当9-OHFLU与金电极上的发夹DNA膜相互作用时，发夹DNA膜的电荷转移电阻（R-CT）显著增加，并在30 min后保持恒定。根据9-OHFLU与发夹DNA相互作用前后的电荷转移电阻（Delta R-CT）的差异，可以在低至1 nM的浓度下检测到9-OHFLU。然而，8种羟基多环芳烃（HO-PAHs）与发夹DNA作用30 min后，只有较小的Δ R-CT值出现，表明9-OHFLU可以通过EIS与其他HO-PAHs区分开来。在真实的湖水样品中进一步探索了传感器的性能，用于检测9-OHFLU，检测限为4 nM。
The direct interaction of hairpin DNA with 9-hydroxyfluorene (9-OHFLU) through hydrogen bonds was investigated by electrochemical impedance spectroscopy (EIS), UV-Vis spectroscopy and H-1 NMR spectra. Based on these results, an electrochemical hairpin DNA sensor was developed for the detection of 9-OHFLU by EIS. Upon 9-OHFLU interacting with hairpin DNA film on the gold electrodes, the charge transfer resistance (R-CT)of the hairpin DNA film was significantly increased and remained constant after 30 min. Depending on the difference in charge transfer resistance (Delta R-CT) before and after 9-OHFLU interaction with the hairpin DNA, 9-OHFLU could be detected with a concentration as low as 1 nM. However, only a much smaller Delta R-CT appeared when eight selected hydroxyl polycyclic aromatic hydrocarbons (HO-PAHs) interacted with the hairpin DNA for 30 min, which demonstrated that 9-OHFLU could be discriminated from other HO-PAHs by EIS. The performance of the sensor in real lake water sample was further explored for the detection of 9-OHFLU with the detection limit of 4 nM.