Enzymatic Janus Liposome Micromotors
Enzymatic Janus Liposome Micromotors
复制标题
酶促 Janus 脂质体微电机
DOI：
10.1021/acs.langmuir.3c00335
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发表时间：
2023
期刊：
影响因子：
3.9
通讯作者：
Zhan, Wei
中科院分区：
文献类型：
--
作者：
Jin, Hui;Cui, Jinyan;Zhan, Wei
提出了一种基于脂质体的微电机系统，该系统利用区域酶转化和气体产生来实现在水中的定向运动。这些脂质体主要由低熔点脂质和高熔点脂质以及胆固醇组成，由于脂质液-液相分离，这些脂质体在室温下保持稳定的Janus构型。酶如辣根过氧化物酶的局部放置是通过亲和素和生物素之间的亲和结合实现的，后者作为脂质缀合物特异性地分选到这些Janus脂质体的一个结构域中作为次要组分。在底物过氧化氢的存在下，这些酶修饰的Janus脂质体进行定向运动，在某些情况下产生超过热扩散三倍的速度。脂质体的大小控制，电机组装，和基板分布的实验细节;脂质体运动的关键实验因素，如基板浓度和脂质体Janus比的影响，也进行了检查。因此，这项工作提供了一个可行的方法来建立不对称的脂质组装，酶连接的胶体，此外，强调的重要性，实现粒子定向运动的不对称性。
A liposome-based micromotor system that utilizes regional enzymatic conversion and gas generation to achieve directional motion in water is presented. Constituted mainly of a low-melting lipid and a high-melting lipid together with cholesterol, these liposomes maintain stable Janus configuration at room temperature as a result of lipid liquid–liquid phase separation. Local placement of enzymes such as horseradish peroxidase is realized via affinity binding between avidin and biotin, the latter as a lipid conjugate sorted specifically into one domain of these Janus liposomes as a minor component. In the presence of the substrate, hydrogen peroxide, these enzyme-decorated Janus liposomes undergo directional motion, yielding velocities exceeding thermal diffusion by three folds in some cases. Experimental details on liposome size control, motor assembly, and substrate distribution are presented; effects of key experimental factors on liposome motion, such as substrate concentration and liposome Janus ratio, are also examined. This work thus provides a viable approach to building asymmetrical lipid-assembled, enzyme-attached colloids and, in addition, stresses the importance of asymmetry in achieving particle directional motion.