Immunological resistance to pulmonary metastases in C3Hf-Bu mice bearing syngeneic fibrosarcoma of different sizes.
Immunological resistance to pulmonary metastases in C3Hf-Bu mice bearing syngeneic fibrosarcoma of different sizes.
复制标题
携带不同大小同基因纤维肉瘤的 C3Hf-Bu 小鼠对肺转移的免疫抵抗。
作者：
Luka Milas;N. Hunter;Kathy Mason;H. Withers
摘要 在 C3Hf/Bu 小鼠中研究了对同基因甲基胆蒽诱导的纤维肉瘤 (FSA) 肺转移的免疫抵抗力，这些小鼠主动免疫或携带该肿瘤不同时间。通过静脉注射诱导肿瘤转移。接种FSA细胞，14至17天后测定其数量。在先前针对同一肿瘤进行免疫的小鼠中，转移的数量大大减少，但在针对同基因乳腺癌免疫的小鼠中则没有。这种减少是通过免疫脾和淋巴结细胞的活性介导的，并且与转移到受体小鼠中的免疫细胞的数量有关。发现脾细胞比淋巴结细胞更有效。皮下生长肿瘤的存在减少了肺转移的数量。这是在 s.c. 时注意到的。 FSA 植入先于静脉注射。在3小时和3、7、特别是14天时接种肿瘤细胞。来自携带皮下生长的 FSA 3、8、12、20 和 27 天的小鼠的脾细胞能够将免疫力转移到亚致死辐射的同基因受体中的 FSA 肺转移灶。荷瘤小鼠脾细胞持续 12 天，可最有效地转移免疫；在携带肿瘤27天的小鼠中，脾细胞的这种活性显着下降。然而，只有从携带肿瘤12或20天的小鼠体内获取淋巴结细胞，才能转移抗肿瘤免疫力。静脉注射携带 FSA 的小鼠血清对肿瘤细胞受体小鼠进行单次或多次注射并不能阻止免疫脾细胞和淋巴结细胞减少肺转移。
Summary Immunological resistance to pulmonary metastases of a syngeneic methylcholanthrene-induced fibrosarcoma (FSA) was studied in C3Hf/Bu mice that were actively immunized against or that had borne that tumor for varying times. Tumor metastases were induced by i.v. inoculation of FSA cells, and their number was determined 14 to 17 days later. The number of metastases was greatly reduced in mice that previously had been immunized against the same tumor but not in those immunized against syngeneic mammary carcinoma. This reduction was mediated through the activity of immune spleen and lymph node cells and was related to the number of immune cells transferred into the recipient mice. Spleen cells were found to be more effective than lymph node cells. The presence of the s.c.-growing tumor reduced the number of pulmonary metastases. This was noted when s.c. implantation of FSA preceded the i.v. inoculation of the tumor cells by 3 hr and 3, 7, and particularly 14 days. Spleen cells from mice bearing s.c.-growing FSA for 3, 8, 12, 20, and 27 days were able to transfer immunity to pulmonary metastases of FSA in sublethally irradiated syngeneic recipients. Immunity was transferred most effectively by spleen cells from mice bearing the tumor for 12 days; this activity of the spleen cells markedly decreased in mice bearing the tumor for 27 days. Lymph node cells, however, were able to transfer antitumor immunity only if taken from mice bearing the tumors for 12 or 20 days. Serum from mice bearing FSA given i.v. to the tumor cell recipient mice as a single injection or in multiple doses did not prevent the reduction of pulmonary metastases by immune spleen and lymph node cells.