Comparative proteomics of leaves found at different stem positions of maize seedlings
Comparative proteomics of leaves found at different stem positions of maize seedlings
复制标题
玉米幼苗不同茎位置叶片的比较蛋白质组学
DOI：
10.1016/j.jplph.2016.03.022
复制
发表时间：
2016-07-01
影响因子：
4.3
通讯作者：
Wang, Bai-Chen
中科院分区：
文献类型：
--
作者：
Chen, Yi-Bo;Wang, Dan;Wang, Bai-Chen
为了更好地了解不同茎位叶片在植物发育过程中的作用，我们测量了玉米幼苗茎上第1-4叶的生理特性，并进行了蛋白质组学研究，以探讨蛋白质表达的差异，在四个叶片中使用二维差异凝胶电泳和串联质谱结合数据库搜索。共发现并鉴定了167个显著差异表达的蛋白质点。其中35%参与光合作用。通过对数据的进一步分析，我们推测，在叶1中，幼苗已经开始从异养向自养转变，叶2的发育是幼苗从异养向自养完全转变的时间，并且叶成熟仅在完全展开的叶3和4时达到，尽管在两片叶中仍然存在一些蛋白质表达差异。这些结果表明，不同叶片通过调节光合蛋白的表达对玉米幼苗的生长做出不同的贡献。总而言之，这些结果提供了对不同玉米叶片在植物从异养生物发育到自养生物过程中的作用的深入了解。(C)2016 Elsevier GmbH. All rights reserved.
To better understand the roles of leaves at different stem positions during plant development, we measured the physiological properties of leaves 1-4 on maize seedling stems, and performed a proteomics study to investigate the differences in protein expression in the four leaves using two-dimensional difference gel electrophoresis and tandem mass spectrometry in conjunction with database searching. A total of 167 significantly differentially expressed protein spots were found and identified. Of these, 35% are involved in photosynthesis. By further analysis of the data, we speculated that in leaf 1 the seedling has started to transition from a heterotroph to an autotroph, development of leaf 2 is the time at which the seedling fully transitions from a heterotroph to an autotroph, and leaf maturity was reached only with fully expanded leaves 3 and 4, although there were still some protein expression differences in the two leaves. These results suggest that the different leaves make different contributions to maize seedling growth via modulation of the expression of the photosynthetic proteins. Together, these results provide insight into the roles of the different maize leaves as the plant develops from a heterotroph to an autotroph. (C) 2016 Elsevier GmbH. All rights reserved.