CD49f-based selection of circulating tumor cells (CTCs) improves detection across breast cancer subtypes
CD49f-based selection of circulating tumor cells (CTCs) improves detection across breast cancer subtypes
复制标题
DOI：
10.1016/j.canlet.2011.12.031
复制
发表时间：
2012-06-01
期刊：
影响因子：
9.7
通讯作者：
Sleijfer, Stefan
中科院分区：
文献类型：
--
作者：
Mostert, Bianca;Kraan, Jaco;Sleijfer, Stefan
循环肿瘤细胞（CTC）可以使用CellSearch计数，但并非所有乳腺癌亚型，特别是具有上皮-间质转化（EMT）特征的那些，都充分表达该方法中使用的富集（EpCAM）和选择（CK 8/18/19）标志物。虽然CD 146可以检测EpCAM阴性CTC，但我们在此评估了各种细胞角蛋白和CD 49 f检测CK 8/18/19阴性CTC的价值。所测试的细胞角蛋白没有提供实质性的益处，但是将CD 49 f作为选择标记物添加到CK 8/18/19导致改善的正常样细胞系的恢复。(c)2011爱思唯尔爱尔兰有限公司保留所有权利。
Circulating tumor cells (CTCs) can be enumerated using CellSearch, but not all breast cancer subtypes, specifically those with epithelial-mesenchymal transition (EMT) characteristics, sufficiently express the enrichment (EpCAM) and selection (CK8/18/19) markers used in this method. While CD146 can detect EpCAM-negative CTCs, we here evaluated the value of various cytokeratins and CD49f to detect CK8/18/19-negative CTCs. The tested cytokeratins provided no substantial benefit, but adding CD49f to CK8/18/19 as a selection marker resulted in improved recovery of normal-like cell lines.Combined staining of CK8/18/19 and CD49f after CD146/EpCAM enrichment is likely to further improve CTC detection in breast cancer. (c) 2011 Elsevier Ireland Ltd. All rights reserved.