Evolution of repetitive proteins:: spider silks from Nephila clavipes (Tetragnathidae) and Araneus bicentenarius (Araneidae)
Evolution of repetitive proteins:: spider silks from Nephila clavipes (Tetragnathidae) and Araneus bicentenarius (Araneidae)
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DOI：
10.1016/s0965-1748(97)00083-0
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发表时间：
1998-03-01
影响因子：
3.8
通讯作者：
Stote, R
中科院分区：
文献类型：
--
作者：
Beckwitt, R;Arcidiacono, S;Stote, R
蜘蛛丝是高度重复的蛋白质，其特征在于富含聚丙氨酸和甘氨酸的重复单元的区域。我们已经获得了两个变种的蜘蛛丝蛋白（NCF-1）的丝绸基因序列从Nephila clavipes。一个序列（1726 bp）来自克隆的cDNA，另一个序列（1951 bp）来自基因组DNA的PCR。当将这些序列彼此比较并与先前公布的蛛丝蛋白1序列比较时，由于序列重排以及单碱基取代而存在差异。这些变异与其他高度重复基因的变异相似，可能是不平等交换的结果。对双棘园蛛基因组DNA进行PCR扩增，获得了708 bp的序列。该序列与A. diadematus的Spidroin 2（NCF-2）和N.锁骨。A.在重复单元序列中存在微小但一致的差异。bicentenarius和A. diadematus表明协同进化或基因转换过程的作用是保持单一基因内重复单元之间的相似性。(C)1998爱思唯尔科技有限公司版权所有。
Spider silks are highly repetitive proteins, characterized by regions of polyalanine and glycine-rich repeating units. We have obtained two variants of the Spidroin (NCF-1) silk gene sequence from Nephila clavipes. One sequence (1726 bp) was from a cloned cDNA, and the other (1951 bp) was from PCR of genomic DNA. When these sequences are compared with each other and the previously published Spidroin 1 sequence, there are differences due to sequence rearrangements, as well as single base substitutions. These variations are similar to those that have been reported from other highly repetitive genes, and probably represent the results of unequal cross-overs. We have also obtained 708 bp of sequence from PCR of genomic DNA from Araneus bicentenarius. This sequence shows considerable similarity to a dragline sequence (ADF-3) from A. diadematus, as well as Spidroin 2 (NCF-2) from N. clavipes. Minor but consistent differences in the repeating unit sequence between A. bicentenarius and A. diadematus suggest that concerted evolution or gene conversion processes are acting to maintain similarity among repeat units within a single gene. (C) 1998 Elsevier Science Ltd. All rights reserved.