Molecular organization of the lipid matrix in intact Stratum corneum using ATR-FTIR spectroscopy
Molecular organization of the lipid matrix in intact Stratum corneum using ATR-FTIR spectroscopy
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DOI：
10.1016/j.bbamem.2008.01.022
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发表时间：
2008-05-01
影响因子：
3.4
通讯作者：
Normand, Valery
中科院分区：
文献类型：
--
作者：
Boncheva, Mila;Damien, Fabienne;Normand, Valery
ATR-FTIR光谱用于研究全层皮肤角质层（SC）脂质的横向组织。基于对正三聚糖和人体皮肤的热致性相变的研究，CH2剪切带宽的温度依赖性作为正交和六方相程度的测量。与基于差谱、CH2剪切区曲线拟合和CH2拉伸振动位置的常见光谱方法相比，这种依赖关系提供了一种更简单的脂质组装横向顺序测量方法。它具有易于测定、相对较低的变异性和对分子间侧链填料类型判别能力强的优点。利用剪切带宽对哺乳动物皮肤SC表面脂质横向组织进行比较，发现人类腹部皮肤（主要含正交相）、猪耳皮肤（主要含六角形相）和重建的人类表皮（主要含无序相）之间存在相当大的差异。该参数也正确地描述了丙二醇（最小干扰）和油酸（形成高度无序相）对切除人体皮肤SC脂质的不同影响。这里描述的程序适用于皮肤病学、经皮给药和皮肤生物物理学领域的体内研究。(C) 2008 Elsevier B.V.版权所有
ATR-FTIR spectroscopy is useful in investigating the lateral organization of Stratum corneum (SC) lipids in full-thickness skin. Based on studies of the thermotropic phase transitions in n-tricosane and in excised human skin, the temperature dependence of the CH2 scissoring bandwidth emerged as a measure of the extent of orthorhombic and hexagonal phases. This dependence provides a simpler measure of the lateral order in lipid assemblies than the common spectroscopic approaches based on difference spectra, curve fitting of the CH2 scissoring region, and the position of the CH2 stretching vibrations. It has the advantages of ease of determination, relatively low variability, and high discriminative power for the type of lateral intermolecular chain packing. A comparison of the lateral organization of the lipids at the SC surface of mammalian skin using the scissoring bandwidth revealed considerable differences between human abdominal skin (containing mostly orthorhombic phases), porcine ear skin (containing mostly hexagonal phases), and reconstructed human epidermis (containing mostly disordered phases). This parameter also correctly described the different effects of propylene glycol (minimally disturbing) and oleic acid (formation of a highly disordered phase) on the SC lipids in excised human skin. The procedure described here is applicable to in vivo studies in the areas of dermatology, transdermal drug delivery, and skin biophysics. (C) 2008 Elsevier B.V. All rights reserved.