Chlamydomonas reinhardtii
Chlamydomonas reinhardtii
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DOI：
10.1385/mb:34:2:213
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发表时间：
2006-10-01
影响因子：
2.6
通讯作者：
Heitzer, Markus
中科院分区：
文献类型：
--
作者：
Griesbeck, Christoph;Kobl, Iris;Heitzer, Markus
重组蛋白在制药和化工行业中的地位越来越重要。尽管已经开发了各种蛋白质表达系统，但对廉价的大规模生产方法的需求越来越大。真核藻类可以作为生产重组蛋白的一种新选择，因为它们可以以廉价和简单的方式培养，并生长到高细胞密度。作为一种模式生物，单细胞绿色衣藻莱茵衣藻在过去的几十年里得到了深入的研究，现在提供了一个完整的工具，遗传操作。最近，已经报道了几种具有药物相关性的蛋白质的成功表达，从核和叶绿体基因组的这种植物，证明了其生物技术应用的能力。
Recombinant proteins have become more and more important for the pharmaceutical and chemical industry. Although various systems for protein expression have been developed, there is an increasing demand for inexpensive methods of large-scale production. Eukaryotic algae could serve as a novel option for the manufacturing of recombinant proteins, as they can be cultivated in a cheap and easy manner and grown to high cell densities. Being a model organism, the unicellular green alga Chlamydomonas reinhardtii has been studied intensively over the last decades and offers now a complete toolset for genetic manipulation. Recently, the successful expression of several proteins with pharmaceutical relevance has been reported from the nuclear and the chloroplastic genome of this alga, demonstrating its ability for biotechnological applications.