Characterization of the transient species in the 266-nm laser photolysis of adenine and its derivatives.
Characterization of the transient species in the 266-nm laser photolysis of adenine and its derivatives.
复制标题
腺嘌呤及其衍生物的 266 nm 激光光解中瞬态物质的表征。
DOI：
10.1111/j.1751-1097.1987.tb07872.x
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发表时间：
1987
影响因子：
3.3
通讯作者：
Arce,R
中科院分区：
文献类型：
--
作者：
Arce,R
在不同pH条件下测量了腺嘌呤、2'-脱氧腺苷、2'-脱氧腺苷-5'-磷酸、腺苷-5'-磷酸、2'-脱氧腺苷基-(3'-5')-2-脱氧腺苷、7-甲基腺嘌呤和9-甲基腺嘌呤水溶液的266 nm ns激光闪光光解产生的瞬态吸收光谱。激发后观察到的瞬态主要由水合电子、自由基阳离子、自由基阴离子和/或由其质子化产生的中性自由基组成，以及来自激发三重态的次要贡献。光电离主要通过激发单线态发生。对于腺嘌呤，使用激光和脉冲辐射分解技术测定阳离子和阴离子自由基吸收光谱。通过敏化实验确定了三重态的存在。水合电子在与中性碱发生伪一级反应后衰变。
Transient absorption spectra produced by 266‐nm ns laser flash photolysis of aqueous solutions of adenine, 2′‐deoxyadenosine, 2′‐deoxyadenosine‐5′‐phosphate, adenosine‐5′‐phosphate, 2′‐deoxyadenylyl‐(3′‐5′)‐2‐deoxyadenosine, 7‐methyladenine and 9‐methyladenine have been measured under different pH conditions. The transients observed after excitation consisted mainly of the hydrated electron, the radical cation, the radical anion and/or neutral radicals resulting from its protonation, and a minor contribution from the excited triplet state. The photoionization occurs mainly through an excited singlet state. For adenine, the cation and anion radical absorption spectra were determined using laser and pulse radiolysis techniques. The triplet state presence was established through sensitization experiments. The hydrated electron decays following a pseudo‐first‐order reaction with the neutral base.