Short catkin 1, a novel mutant of Castanea mollissima, is associated with programmed cell death during chestnut staminate flower differentiation
Short catkin 1, a novel mutant of Castanea mollissima, is associated with programmed cell death during chestnut staminate flower differentiation
复制标题
DOI：
10.1016/j.scienta.2011.07.014
复制
发表时间：
2011-09-14
影响因子：
4.3
通讯作者：
Han, Zhen-hai
中科院分区：
文献类型：
--
作者：
Feng, Yong-qing;Shen, Yuan-yue;Han, Zhen-hai
板栗雄花数量远多于雌花数量是限制板栗产量的一个因素。在中国北京附近山区的一棵板栗树的一个分支上发现了一个自然发生的雄性柔荑花序突变。该突变被命名为短catkin 1（sck 1）。sck 1的雄花序长度仅为野生型雄花序的1/6 ~ 1/8。该突变与更多的雌花和产量增加有关。对sck 1型雄花序发育过程的观察表明，雄花序在雄花分化期顶端败育。透射电镜分析表明，sck 1柔荑花序的细胞死亡过程在亚细胞水平上具有典型的细胞程序性死亡特征，如染色质浓缩、核仁溶解、核质降解、核膜破裂等。和解体的叶绿体或线粒体sck 1的花序组织中存在明显的DNA梯状条带。结果表明，sck 1与PCD相关。(C)2011 Elsevier B. V.保留所有权利。
The fact that male flowers far outnumber female flowers is a factor that limits nut yield in Chinese chestnut. A naturally occurring mutation of male catkins was found on a single branch of a Chinese chestnut tree in the mountains near Beijing, China. The mutation was named short catkin1 (sck1). The catkin length of sck1 was only 1/6 to 1/8 that of the wild-type male catkins on the same tree. The mutation was associated with a greater number of female flowers and increased yield. Observations on the development of male catkins with the sck1 phenotype showed that the distal part of the catkins aborted at the stage of chestnut staminate flower differentiation. Further research using transmission electron microscope analysis showed that the process of cell death in sck1 catkins had the typical characteristics of programmed cell death at the subcellular level, such as condensed chromatin, dissolved nucleolus, degraded karyplasm, burst karyotheca. and disintegrated chloroplasts or mitochondria. Significantly, DNA laddering was detected in tissues of sck1 catkins. In conclusion, the results showed that sck1 was associated with PCD. (C) 2011 Elsevier B.V. All rights reserved.