VEGF and ki 67 expression in squamous cell carcinoma of the tongue: An immunohistochemical and computerized image analysis study
VEGF and ki 67 expression in squamous cell carcinoma of the tongue: An immunohistochemical and computerized image analysis study
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DOI：
10.1016/j.oraloncology.2008.08.002
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发表时间：
2009-07-01
期刊：
影响因子：
4.8
通讯作者：
Patsouris, Efstratios
中科院分区：
文献类型：
--
作者：
Faratzis, Gregory;Tsiambas, Evangelos;Patsouris, Efstratios
Ki-67和血管内皮生长因子（VEGF）的过度表达是口腔粘膜鳞状细胞癌（SCC）中常见的现象。VEGF和ki 67蛋白的表达进行了研究，在一个队列的87例原发性，未经治疗的舌鳞状细胞癌，使用计算机图像分析（CIA），以确定这些因素的潜在预后意义。用单克隆抗ki 67（MIB 1）和抗VEGF抗体进行免疫组织化学分析。应用数字图像分析测定来评价结果。使用CIA，在24/87（27.5%）的检查病例中观察到VEGF过表达，这一发现与疾病的分期相关（p = 0.05）。Ki 67在49/87例（56.3%）病例中过表达，并与肿瘤大小相关（p = 0.05）。考克斯回归分析显示，VEGF蛋白表达与受检患者的生存状态无预后意义（p = 0.77），而ki 67过表达与不良预后密切相关（p = 0.017）。原发性肿瘤的大小也与患者的生存状态密切相关（p = 0.024），而疾病分期显示出边缘统计学显著性（p = 0.091）。(C)2008爱思唯尔有限公司保留所有权利。
Over-expression of ki 67 and vascular endothelial growth factor (VEGF) is a frequent finding in squamous cell carcinoma (SCC) of the oral mucosa. The expression of VEGF and ki 67 proteins was studied in a cohort of 87 patients with primary, previously untreated SCC of the tongue, using computerized image analysis (CIA) in order to determine the potential prognostic significance of these factors. Immunohistochemical analysis was performed with monoclonal anti-ki 67 (MIB 1) and anti-VEGF antibodies. A digital image analysis assay was applied for the evaluation of the results. Using CIA, VEGF over-expression was observed in 24/87 (27.5%) of the examined cases and this finding correlated to the stage of the disease (p = 0.05). ki 67 was over-expressed in 49/87 (56.3%) of the cases and correlated to the size of the tumors (p = 0.05). Cox regression analysis showed that there was no prognostic significance associating VEGF protein expression to survival status of the examined patients (p = 0.77), whereas ki 67 over-expression was strongly correlated to poor prognosis (p = 0.017). The size of the primary tumors was also strongly correlated to survival status of the patients (p = 0.024), whereas stage of disease showed a borderline statistical significance (p = 0.091). (C) 2008 Elsevier Ltd. All rights reserved.