EVIDENCE OF A NOVEL ASSOCIATION OF UNSATURATED FATTY-ACIDS WITH GC (VITAMIN-D-BINDING PROTEIN)
EVIDENCE OF A NOVEL ASSOCIATION OF UNSATURATED FATTY-ACIDS WITH GC (VITAMIN-D-BINDING PROTEIN)
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DOI：
10.1016/s0006-291x(88)81330-5
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发表时间：
1988-06-30
影响因子：
3.1
通讯作者：
GALBRAITH, RM
中科院分区：
文献类型：
--
作者：
WILLIAMS, MH;VANALSTYNE, EL;GALBRAITH, RM
最近描述的Gc（维生素D结合蛋白）和白蛋白之间的强序列同源性，以及后者结合2-对甲苯胺基萘磺酸盐（TNS）的能力，促使与Gc类似的结合研究。TNS结合天然Gc的荧光，但氯仿：甲醇提取的Gc和气相色谱显示，大量的不饱和脂肪酸- 16：1，18：1，18：2和20：4也与Gc。此外，TNS荧光被取消脱脂的GC，并恢复后，随后重建与脂肪酸。最后，将70-80%的[3 H]-花生四烯酸加入到全血清中，与GC结合。这些结果表明，TNS荧光的天然分子反映了相关的脂质，并提出了一个新的作用，Gc作为一个水库的循环池的不饱和脂肪酸。
The strong sequence homology described recently between Gc (Vitamin D-binding protein) and albumin, and the ability of the latter, to bind 2-p-toluidinylnaphthylene sulfonate (TNS) prompted similar binding studies with Gc. TNS bound to native Gc as demonstrated by fluorescence, but chloroform:methanol extraction of Gc and gas chromatography revealed that large amounts of unsaturated fatty acids - 16:1, 18:1, 18:2 and 20:4 were also associated with Gc. In addition, TNS fluorescence was abolished by delipidation of Gc, and restored upon subsequent reconstitution with fatty acid. Finally, 70-80% of [3H]-arachidonic acid added to whole serum bound to Gc. These results demonstrated that TNS florescence of the native molecule reflects associated lipid, and suggest a novel role for Gc as a reservoir for a circulating pool of unsaturated fatty acids.