Miniature Swine as a Clinically Relevant Model of Graft-Versus-Host Disease.
Miniature Swine as a Clinically Relevant Model of Graft-Versus-Host Disease.
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DOI：
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发表时间：
2015-10
影响因子：
0.8
通讯作者：
R. Duran‐Struuck;Christene A. Huang;Katherine Orf;R. Bronson;D. Sachs;T. Spitzer
中科院分区：
文献类型：
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作者：
R. Duran‐Struuck;Christene A. Huang;Katherine Orf;R. Bronson;D. Sachs;T. Spitzer
小型猪为移植物抗宿主病的研究提供了一种造血细胞移植的临床前模型。可以在mhc匹配或不匹配的猪之间进行HCT，以模拟临床情景，其结果与在人类HCT受体中观察到的结果非常相似。在清髓条件下，跨越MHC屏障的HCT通常是致命的，猪发展为严重的（III级或IV级）GVHD，涉及胃肠道、肝脏和皮肤。与啮齿类动物模型不同，微型猪提供了对单个动物进行长期纵向研究的机会，因为可以在不需要安乐死的情况下进行多个组织活检。此外，我们已经开发了一种猪GVHD评分系统，与人类临床环境中使用的系统相似。鉴于猪和人类GVHD的相似性，我们希望使用该评分系统促进实验室和临床之间的临床和科学论述。我们预计猪的研究结果将支持开发新的策略，以改善临床HCT中GVHD的识别和治疗。
Miniature swine provide a preclinical model of hematopoietic cell transplantation (HCT) for studies of graft-versus-host disease. HCT between MHC-matched or -mismatched pigs can be performed to mimic clinical scenarios with outcomes that closely resemble those observed in human HCT recipients. With myeloablative conditioning, HCT across MHC barriers is typically fatal, with pigs developing severe (grade III or IV) GVHD involving the gastrointestinal tract, liver, and skin. Unlike rodent models, miniature swine provide an opportunity to perform extended longitudinal studies on individual animals, because multiple tissue biopsies can be harvested without the need for euthanasia. In addition, we have developed a swine GVHD scoring system that parallels that used in the human clinical setting. Given the similarities of GVHD in pigs and humans, we hope that the use of this scoring system facilitates clinical and scientific discourse between the laboratory and the clinic. We anticipate that results of swine studies will support the development of new strategies to improve the identification and treatment of GVHD in clinical HCT scenarios.