Effect of chemical inactivating agents on glucocorticoid receptor proteins in mouse and hamster cells.
Effect of chemical inactivating agents on glucocorticoid receptor proteins in mouse and hamster cells.
复制标题
化学灭活剂对小鼠和仓鼠细胞中糖皮质激素受体蛋白的影响。
DOI：
10.1073/pnas.72.8.3060
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发表时间：
1975
影响因子：
11.1
通讯作者：
E. Scolnick
中科院分区：
文献类型：
--
作者：
H. Young;W. Parks;E. Scolnick
本文研究了N-乙基马来酰亚胺和碘乙酰胺对从小鼠或仓鼠细胞胞浆中提取的糖皮质激素受体活性的影响。用N-乙基马来酰亚胺或碘乙酰胺处理小鼠或仓鼠胞质溶胶可迅速灭活任一胞质溶胶的[3 H]糖皮质激素结合活性。糖皮质激素地塞米松与胞质受体的预结合阻断了N-乙基马来酰亚胺对受体的快速失活。用碘乙酰胺处理预结合的酶-受体复合物可防止酶-受体复合物随后与DNA结合，而不引起复合物解离。虽然结论可能是有限的缺乏纯度的受体，结果表明，巯基参与糖皮质激素受体蛋白的结合。此外，结果表明，碘乙酰胺是失活的一个单独的化学位点，这是必要的结合酶受体复合物的DNA。
The ffect of N-ethylmaleimide and iodoacetamide on the glucocorticoid receptor activity extracted from the cytosol of either mouse of hamster cells has been investigated. Treatment of mouse or hamster cytosol with N-ethylmaleimide or iodoacetamide rapidly inactivates the [3H]glucocorticoid hormone binding activity of either cytosol. Prebinding the glucocorticoid hormone, dexamethasone, to the cytosol receptor blocks the rapid inactivation of the receptor by N-ethylmaleimide. Treatment of the prebound hormone-receptor complex with iodoacetamide prevents the subsequent binding of the hormone-receptor complex to DNA without causing a dissociation of the complex. Although the conclusions may be limited by the lack of purity of the receptor, the results suggest that a sulfhydryl group is involved in the binding of glucocorticoid hormones to the receptor protein. In addition, the results suggest that iodoacetamide is inactivating a separate chemical site which is necessary for the binding of the hormone-receptor complex to DNA.