Schwann cells can induce collateral sprouting from intact axon: Experimental study of end-to-side neurorrhaphy using a Y-chamber model
Schwann cells can induce collateral sprouting from intact axon: Experimental study of end-to-side neurorrhaphy using a Y-chamber model
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DOI：
10.1055/s-2007-1000102
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发表时间：
1999-05-01
影响因子：
2.1
通讯作者：
Uchida, A
中科院分区：
文献类型：
--
作者：
Matsumoto, M;Hirata, H;Uchida, A
最近报道了端侧神经缝合术中的轴突再生；然而，完整轴突侧枝萌发的机制以及再生轴突的起源尚不清楚。甚至对于完整轴突是否存在侧支轴突萌芽也存在争议。本报告的实验研究旨在澄清这些问题。使用大鼠坐骨神经模型。为了避免在手术过程中对供体神经造成任何机械损伤，采用 Y 形硅胶腔代替直接缝合。使用逆行神经示踪剂和免疫组织化学染色评估从完整胫神经穿过间隙进入腓神经的轴突再生。 66% 的动物观察到跨间隙的轴突再生。神经示踪剂评估清楚地表明所有再生轴突都是来自背根神经节的感觉轴突。作者得出的结论是，来自神经节段远端华勒变性的施万细胞确实引发了完整感觉轴突的侧支轴突发芽，但端侧神经缝合术中的运动轴突则没有。雪旺细胞侵入神经外膜层是完整轴突开始侧支轴突发芽的关键步骤。
Axonal regeneration across end-to-side neurorrhaphy has recently been reported; however, neither the mechanism by which collateral sprouting from intact axons is elicited, nor the origin of the regenerating axons are known. There has even been controversy over the presence of collateral axonal sprouting from intact axons altogether. This reported experimental study was designed to clarify these questions. A rat sciatic nerve model was used. To avoid any mechanical damage to the donor nerve during the procedure, a Y-shaped silicone chamber was employed instead of direct suture. Axonal regeneration from the intact tibial nerve across the gap into the peroneal nerve was assessed using a retrograde neurotracer and immunohistochemical staining. Axonal regeneration across the gap was observed in 66 percent of the animals. The neurotracer evaluation clearly showed that all regenerating axons were sensory axons from the dorsal root ganglia. The authors concluded that Schwann cells from the distal wallerian degeneration of nerve segments did elicit collateral axonal sprouting from intact sensory axons, but not from motor axons in end-to-side neurorrhaphy. Invasion of the Schwann cells into the epineurial layer was the crucial step for the initiation of collateral axonal sprouting from the intact axons.