New furostanol saponins from Smilax aspera L. and their in vitro cytotoxicity
New furostanol saponins from Smilax aspera L. and their in vitro cytotoxicity
复制标题
DOI：
10.1016/j.fitote.2010.10.012
复制
发表时间：
2011-03-01
期刊：
影响因子：
3.4
通讯作者：
Kostova, Ivanka
中科院分区：
文献类型：
--
作者：
Ivanova, Antoaneta;Mikhova, Bozhanka;Kostova, Ivanka
两个新的顺式稠合A/B环呋喃甾醇皂苷(25S)-26-O-β-D-吡喃葡萄糖基-5-β-呋喃-1β，3-β，22α，26-四醇-1-O-β-D-吡喃葡萄糖苷和(25S)-26-O-β-D-吡喃葡萄糖-5-β-呋喃-1β，2β，3-β，5-β，22α，26-己醇及其已知化合物(25S)-26-O-β-D-吡喃葡萄糖基-5β-呋喃-3β，22，α，26-己醇26-三醇-3-O-α-L-鼠李糖基-(1->；2)-O-β-D-吡喃葡萄糖基-(1-gt；2)-O-β-D-吡喃葡萄糖苷和(25S)-26-O-β-D-吡喃葡萄糖基-5-β-呋喃-3-β，22α，26-三醇-3-O-β-D-吡喃葡萄糖苷-(1->；2)-O-β-D-吡喃葡萄糖苷、反式白藜芦醇、(+)儿茶素和(-)表儿茶素。所有皂苷均被分离为其22-OME衍生物，并进行了广泛的光谱分析。用中性红法和四甲基偶氮唑盐比色法测定了分离得到的呋喃甾醇皂苷对人正常羊水癌细胞和人肺癌细胞株的细胞毒作用。体外实验显示出明显的细胞毒性，且呈剂量依赖关系，IC50值在32.98-94.53亩M(C)2010 Elsevier B.V.
The occurrence of the two new cis-fused A/B rings furostanol saponins (25S)-26-O-beta-D-glucopyranosyl-5 beta-furostan-1 beta,3 beta,22 alpha,26-tetraol-1-O-beta-D-glucopyranoside and (25S)-26-O-beta-D-glucopyranosyl-5 beta-furostan-1 beta,2 beta,3 beta,5 beta,22 alpha,26-hexaol and the known compounds (25S)-26-O-beta-D-glucopyranosyl-5 beta-furostan-3 beta,22 alpha,26-triol-3-O-alpha-L-rhamnopyranosyl-(1 -> 2)-O-beta-D-glucopyranosyl-(1 -> 2)-O-beta-D-glucopyranoside and (25S)-26-O-beta-D-glucopyranosyl-5 beta-furostan-3 beta,22 alpha,26-triol-3-O-beta-D-glucopyranosyl-(1 -> 2)-O-beta-D-glucopyranoside, trans-resveratrol, (+) catechin and (-) epicatechin in the rhizomes of Smilax aspera is reported. All saponins have been isolated as their 22-OMe derivatives, which were further subjected to extensive spectroscopic analysis. The isolated furostanol saponins were evaluated for cytotoxic activity against human normal amniotic and human lung carcinoma cell lines using neutral red and MTT assays. In vitro experiments showed significant cytotoxicity in a dose dependent manner with IC50 values in the range of 32.98-94.53 mu M. (C) 2010 Elsevier B.V. All rights reserved.