Time course of cholinergic and monoaminergic changes in rat brain after immunolesioning with 192 IgG-saporin.
Time course of cholinergic and monoaminergic changes in rat brain after immunolesioning with 192 IgG-saporin.
复制标题
192 IgG-皂草素免疫损伤后大鼠脑中胆碱能和单胺能变化的时间过程。
DOI：
10.1016/0304-3940(94)90379-4
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发表时间：
1994
影响因子：
2.5
通讯作者：
Thal,LJ
中科院分区：
文献类型：
--
作者：
Waite,JJ;Wardlow,ML;Chen,AC;Lappi,DA;Wiley,RG;Thal,LJ
192 IgG-皂草素（一种针对低亲和力 NGF 受体的免疫毒素）被注入大鼠大脑的侧脑室。病变后三天和一周，皮质、海马、嗅球和隔膜（受基底前脑胆碱能神经元支配的大脑区域）的胆碱乙酰转移酶活性显着降低，而小脑、纹状体或脑桥没有变化。单胺水平的测量显示 HVA、DOPAC 和多巴胺增加，主要仅在 28 天的时间点在嗅球中增加，表明对胆碱能不活动的补偿。使用这种免疫毒素可以获得高水平的基底前脑胆碱能损伤，而对单胺能或其他胆碱能系统影响极小或没有影响。
192 IgG-saporin, an immunotoxin targeted at the low affinity NGF receptor, was infused into the lateral ventricle of rat brain. Three days and one week post lesion, choline acetyltransferase activity was markedly decreased in cortex, hippocampus, olfactory bulbs, and septum (brain regions innervated by the cholinergic neurons of the basal forebrain) with no change in cerebellum, striatum or pons. Measurement of monoamine levels revealed increases in HVA, DOPAC and dopamine, primarily in the olfactory bulbs at the 28-day time point only, suggesting a compensation for cholinergic inactivity. High levels of basal forebrain cholinergic lesioning can be obtained with this immunotoxin with minimal or no effects on monoaminergic or other cholinergic systems.