Multiple, polymorphic copies of SRY in both males and females of the vole Microtus cabrerae
Multiple, polymorphic copies of SRY in both males and females of the vole Microtus cabrerae
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DOI：
10.1159/000134712
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发表时间：
1997-01-01
期刊：
影响因子：
--
通讯作者：
de la Guardia, RD
中科院分区：
文献类型：
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作者：
Bullejos, M;Sánchez, A;de la Guardia, RD
在哺乳动物中，性别决定是由Y连锁基因SRY控制的。虽然SRY在大多数真兽目和有袋类物种中是雄性特异性的，在Y染色体上有一个单拷贝，但几种啮齿类物种有多个Y连锁SRY拷贝，并且两种Ellobius属的鼹鼠物种在没有Y染色体或SRY基因的情况下决定性别。我们在田鼠属的三种田鼠中寻找了SRY的同源基因，并得出结论，该基因在田鼠中不是雄性特异性的。cabrexine，因为它在雄性和雌性中均以多个多态拷贝存在。与此相反，SRY在近缘种M中是雄性特异性的。agrestis和M.尼瓦利斯SRY基因的部分序列在M.咖啡Southern印迹表明，大多数额外的SRY拷贝是X连锁的。仅在雄性中观察到的一个拷贝具有与M中SRY基因相同的序列。Agrestis，可能代表该基因在该物种中的功能拷贝。其余的可能是无功能的假基因。
In mammals, sex determination is controlled by the Y-linked gene SRY. Although SRY is male-specific in most eutherian and marsupial species, with a single copy on the Y chromosome, several rodent species have multiple Y-linked copies of SRY, and two mole-vole species of the genus Ellobius determine sex without the Y chromosome or the SRY gene. We searched for homologs of SRY in three vole species of the genus Microtus and concluded that this gene is not male-specific in M. cabrerae, as it is present in multiple, polymorphic copies in both males and females. In contrast, SRY is male-specific in the related species M. agrestis and M. nivalis. Up to 15 different partial sequences of the SRY gene were found in M. cabrerae. Southern blots suggest that most of the extra copies of SRY are X-linked. One of the copies observed only in males has a sequence identical to that of the SRY gene in M. agrestis and may represent a functional copy of the gene in this species. The rest are probably nonfunctional pseudogenes.