Comparison of insulin autoantibody: polyethylene glycol and micro-IAA 1-day and 7-day assays
Comparison of insulin autoantibody: polyethylene glycol and micro-IAA 1-day and 7-day assays
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DOI：
10.1002/dmrr.1014
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发表时间：
2009-10-01
影响因子：
8
通讯作者：
Eisenbarth, George S.
中科院分区：
文献类型：
--
作者：
Babaya, Naru;Yu, Liping;Eisenbarth, George S.
背景较早的糖尿病发展研究通常使用7天孵育聚乙二醇竞争性胰岛素自身抗体测定（CIAA）。我们的标准微量IAA测定（mIAA）利用沉淀与蛋白质A/G和1天的孵育（1天mIAA），但较不敏感相比，CIAA assay.Methods我们进行了CIAA和mIAA测定在各种条件下。我们分析了来自446名1型糖尿病患者、来自另一组247名开始胰岛素治疗2周内的1型糖尿病患者、来自150名健康对照供体、来自22名年轻糖尿病自身免疫研究（DAISY）的健康参与者的血清样品，结果在研究过程中，发现潜伏期是影响1型糖尿病诊断的关键因素。因此，我们将孵育时间延长至7天（7天mIAA测定）。没有CIAA阴性对照与1天或7天mIAA呈阳性。在一项新发1型糖尿病和高危人群（DAISY研究）中，7天mIAA检测发现另外18%为阳性沿着16%最初1天mIAA阴性和CIAA阳性。大多数受试者检测只有7天的mIAA测定有中间水平的CIAA（80-300 nU/mL）（p = 0.01）。结论7天的mIAA测定确定了一个小的，但显着的额外子集的个人阳性CIAA测定，同时保留特异性。版权所有（C）2009约翰威利父子有限公司
Background Older studies of diabetes development typically utilized a 7-day incubation polyethylene glycol competitive insulin autoantibody assay (CIAA). Our standard micro-IAA assay (mIAA) utilizes precipitation with proteins A/G and 1-day incubation (1-day mIAA), but is less sensitive compared to the CIAA assay.Methods We performed CIAA and mIAA assays in various conditions. We analyzed serum samples from 446 type 1 diabetes patients, from another set of 247 type 1 diabetes patients within 2 weeks of initiation of insulin treatment, from 150 healthy control donors, from 22 healthy participants in the diabetes autoimmunity study in the young (DAISY), and also coded sera from 50 patients with newly diagnosed type 1 diabetes and 50 blood donor control samples.Results In the process of our study, we found that the key condition was the incubation time. Therefore, we extended the incubation time to 7 days (7-day mIAA assay). No CIAA-negative control was positive with either 1-day or 7-day mIAA. In a new onset type 1 diabetes and at risk cohorts (DAISY study), the 7-day mIAA identified an additional 18% as being positive along with 16% of those who were initially 1-day mIAA negative and CIAA positive. Most subjects detectable only with the 7-day mIAA assay had intermediate levels of CIAA (80-300 nU/mL) (p = 0.01).Conclusions The 7-day mIAA assay identifies a small but significant additional subset of individuals positive on the CIAA assay, while preserving specificity. Copyright (C) 2009 John Wiley & Sons, Ltd.