CARBON ASSIMILATION BY PSEUDOMONAS OXALATICUS (OX1) .6. REACTIONS OF OXALYL-COENZYME A
CARBON ASSIMILATION BY PSEUDOMONAS OXALATICUS (OX1) .6. REACTIONS OF OXALYL-COENZYME A
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DOI：
10.1042/bj0870368
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发表时间：
1963-01-01
影响因子：
4.1
通讯作者：
QUAYLE, JR
中科院分区：
文献类型：
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作者：
QUAYLE, JR
通过硫代甲苯基-草酸氢酯和CoA之间的酯交换制备的草酰-CoA是在草酰-辅酶-A还原酶存在下乙醛酸的CoA-和NADP-连接氧化的产物。还原的平衡常数为1.38 × 105（25 ℃）。乙二酰辅酶A和巯基化合物如半胱氨酸和谷胱甘肽之间存在非酶酯交换。这解释了之前不能分离草酰辅酶A的原因。讨论了利用草酰辅酶A还原酶直接分光光度法测定乙醛酸、草酰辅酶A和辅酶A。
Oxalyl-CoA prepared by ester interchange between thiocresyl-hydrogen-oxalate and CoA is the product of CoA- and NADP-linked oxidation of glyoxylate in the presence of oxalyl-coenzyme-A reductase. The equilibrium constant for the reduction is 1.38 X 105 at 25[degree]. There is a nonenzymic ester interchange between oxalyl-CoA and thiol compounds such as cysteine and glutathione. This accounts for the previous inability to isolate oxalyl-CoA. The use of oxalyl-coenzyme-A reductase for the direct spectrophotometric determination of glyoxylate, oxalyl-CoA and CoA is discussed.