Optical measurement of oxygen and temperature in microscale: strategies and biological applications
Optical measurement of oxygen and temperature in microscale: strategies and biological applications
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DOI：
10.1016/s0925-4005(97)80168-2
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发表时间：
1997-01-01
影响因子：
8.4
通讯作者：
Holst, G
中科院分区：
文献类型：
--
作者：
Klimant, I;Kuhl, M;Holst, G
沉积物、微生物垫、生物膜和其他微生物群落的特征在于物理和化学参数的陡峭梯度。微传感器是测量这些参数的强大工具，具有足够的空间分辨率，并对自然系统中的微环境产生很小的干扰。最近，光纤微传感器已被引入水生生物学领域作为现有电化学微传感器的替代品。这种微光电管已经被开发用于溶解氧的高分辨率测量和温度测量。它们易于制造并显示出改善的长期和储存稳定性。概述了不同类型的氧气和温度的微光电管的发展和表征。已经开发了一种基于发光寿命的设备，该设备是便携式的，并且能够在实验室和现场条件下进行微感测。在实际工作中的限制与光学微传感器进行了演示，并简要讨论了克服它们的策略。提出了一种微光电二极管阵列和一种沉积物中高分辨率氧成像的方法，作为两种不同的方法来研究非均匀生物系统中的二维氧分布。最后简要讨论了微光管的应用前景和发展趋势。
Sediments, microbial mats, biofilms and other microbial communities are characterized by steep gradients of physical and chemical parameters. Microsensors are powerful tools to measure these parameters with a sufficient spatial resolution and with a small disturbance of the micro-environment in natural systems. Recently, fiber-optical microsensors have been introduced in the field of aquatic biology as an alternative to existing electrochemical microsensors. Such micro-optodes have already been developed for high-resolution measurement of dissolved oxygen and for temperature measurements. They are easy to fabricate and show an improved long-term and storage stability. An overview is given on the development and characterization of different types of micro-optodes for oxygen and temperature. A luminescence lifetime-based device has been developed which is portable and enables microsensing both in the laboratory and under field conditions. Limitations in practical work with optical microsensors are demonstrated, and strategies to overcome them briefly discussed. A micro-optode array as well as a method for high-resolution oxygen imaging in sediments are presented as two different ways to investigate the two-dimensional oxygen distribution in heterogeneous living systems. Future applications and developments in micro-optode research will be discussed briefly.