Upregulation of MdMYB110a is responsible for ABA-mediated colouration of type 2 red-fleshed apples
Upregulation of MdMYB110a is responsible for ABA-mediated colouration of type 2 red-fleshed apples
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MdMYB110a 的上调导致 ABA 介导的 2 型红肉苹果着色
DOI：
10.1080/14620316.2018.1452638
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Matsumoto S.
中科院分区：
文献类型：
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作者：
Sato H.;Otagaki S.;Ono Y.;Shiratake K.;Matsumoto S.
2型红肉苹果之所以对消费者有吸引力，是因为它具有1型苹果所没有的甜味，此外，它的颜色鲜艳，而且与白苹果相比，其促进健康的多酚化合物的积累程度更高。尽管2型苹果的红色色素沉着是由着色起始时MdMYB110a的表达引起的，但诱导MdMYB110a表达的内源因素却知之甚少。在这项研究中，我们研究了乙烯和脱落酸 (ABA) 两种植物激素对 2 型红肉苹果中 MdMYB110a 表达和果肉色素沉着的影响。尽管乙烯生物合成基因MdACO1表达的诱导与肉色素沉着的开始同时发生，但乙烯或其抑制剂1-甲基环丙烯处理与MdMYB110a的表达模式之间没有发现明显的相关性。然而，苹果中 ABA 诱导基因的同源物在单一果实的色素果肉和非色素果肉之间存在差异表达，并且 2 型红肉果实中的 ABA 处理导致 MdMYB110a 表达上调并增强花青素产量。这些结果强烈表明，ABA（而非乙烯）诱导 MdMYB110a（负责 2 型红肉性状的基因）上调。
Type 2 red-fleshed apples are attractive to consumers due to their sweet taste, which is not present in type 1 apples, in addition to their vivid colour and the accumulation of health-promoting polyphenol compounds to a greater degree than in white apples. Although the red pigmentation of type 2 apples is caused by the expression ofMdMYB110aat colouring initiation, the endogenous factors that induce the expression ofMdMYB110aare poorly understood. In this study, we investigated the effect of two phytohormones, ethylene and abscisic acid (ABA), onMdMYB110aexpression and flesh pigmentation in type 2 red-fleshed apples. Although induction of the expression of the ethylene biosynthetic geneMdACO1coincided with the onset of flesh pigmentation, no obvious correlation was found between treatment with ethylene or its inhibitor 1-methylcyclopropene and the expression pattern ofMdMYB110a. However, homologues of ABA-induced genes in apple were differentially expressed between pigmented and nonpigmented flesh derived from a single fruit, and ABA treatment in type 2 red-fleshed fruits led to the upregulation ofMdMYB110aexpression and the enhancement of anthocyanin production. These results strongly suggest that ABA, not ethylene, induced upregulation ofMdMYB110a, the gene responsible for the type 2 red-flesh trait.