Lack of altered frequency of sister-chromatid exchanges in poly(ADP-ribose) glycohydrolase-deficient mouse ES cells treated with methylmethanesulfonate
Lack of altered frequency of sister-chromatid exchanges in poly(ADP-ribose) glycohydrolase-deficient mouse ES cells treated with methylmethanesulfonate
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DOI：
10.2183/pjab.79b.305
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发表时间：
2003-12-01
影响因子：
3.1
通讯作者：
Masutani, M
中科院分区：
文献类型：
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作者：
Gunji, A;Fujihara, H;Masutani, M
聚（ADP-核糖）糖水解酶在聚（ADP-核糖）降解中起核心作用，切割（核糖-核糖）键以产生ADP-核糖。我们先前通过破坏Parg基因的外显子1产生Parg缺陷（Parg（+/-）和Parg（-/-））小鼠胚胎干（ES）细胞，并显示Parg（-/-）ES细胞对甲基甲磺酸酯（一种烷化剂）高度敏感。为了阐明Parg在维持基因组稳定性中的作用，我们检查了在有和没有甲基甲磺酸酯处理的Parg（+/+）和Parg（-/-）ES细胞中姐妹染色单体交换（SCE）的频率。在Parg基因型之间没有观察到SCE的自发频率的差异，在两种情况下用50 μ M的甲磺酸甲酯治疗引起大约2倍的升高。
Poly(ADP-ribose) glycohydrolase plays a central role in poly(ADP-ribose) degradation, cleaving a(ribose-ribose) bonds to produce ADP-ribose. We previously generated Parg-deficient (Parg(+/-) and Parg(-/-)) mouse embryonic stem (ES) cells by disrupting exon 1 of the Parg gene, and showed the Parg(-/-) ES cells to be hypersensitive to methylmethanesulfonate, an alkylating agent. To clarify the role of Parg in the maintenance of genomic stability, we examined the frequency of sister-chromatid exchanges (SCEs) in Parg(+/+) and Parg(-/-) ES cells with and without methylmethanesulfonate treatment. No difference was observed in the spontaneous frequency of SCEs between the Parg genotypes, treatment with methylmethanesulfonate at 50 muM causing approximately 2-fold elevation in both cases.