Synthesis and Characterization of Vinyl Polymer-Grafted DNA and Its AB-Type Block Conjugate with Native DNA Fragment
Synthesis and Characterization of Vinyl Polymer-Grafted DNA and Its AB-Type Block Conjugate with Native DNA Fragment
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乙烯基聚合物接枝 DNA 及其与天然 DNA 片段的 AB 型嵌段缀合物的合成和表征
DOI：
10.1295/polymj.31.1109
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发表时间：
1999
期刊：
影响因子：
2.8
通讯作者：
M. Maeda
中科院分区：
文献类型：
--
作者：
D. Umeno;Masami Kiji;M. Murata;M. Maeda
我们合成了具有温度响应性和DNA结合能力的聚（N-异丙基丙烯酰胺）（1）和聚（N-异丙基丙烯酰胺-co-N，N-二甲基丙烯酰胺）（2）。通过光化学反应将这些聚合物接枝到质粒双链DNA上。结果表明，接枝了乙烯基聚合物的双链DNA失去了作为RNA合成模板的功能，但表现出乙烯基聚合物特有的溶液性质。聚合物接枝的DNA与天然DNA之间的连接反应产生AB型嵌段缀合物，其中聚合物接枝的DNA为A-片段，天然DNA为B-片段。结果表明，含有两个不同功能结构域的嵌段偶联物保留了两个独立的功能，其中B结构域作为RNA合成的模板，而A结构域对温度有反应，使偶联物沉淀。通过这种方式，人们可以通过使用重组DNA技术，按照自己的意愿构建具有多种功能的定制和精确调控的结构。
We have synthesized psoralen-terminated poly(N-isopropylacrylamide)(1) and poly(N-isopropylacrylamide-co-N,N-dimethylacrylamide)(2), both of which show temperature-responsive properties and DNA-binding ability. These polymers were grafted on plasmid double stranded DNA through a photochemical reaction of their terminal psoralen groups. The double stranded DNA grafted with the vinyl polymers turned out to lose the function as templates for RNA synthesis, while it showed characteristic solution property derived from the vinyl polymers. Ligation reaction between the polymer-grafted DNA and native DNA resulted in the AB-type block conjugate with the polymer-grafted DNA as A-segment and native DNA as B-segment. It was found that the block conjugate which have two different functional domains retains two functions independently; B-domain of the block conjugate worked as a template for RNA synthesis, while A-domain responded to temperature and made the conjugate precipitate. In this way, one can construct tailor-made and precisely-regulated structures with multiple functions at his own will by using recombinant DNA techniques.
DOI：
10.1016/0378-4347(93)80040-b
发表时间：
1993-08
期刊：
Journal of chromatography
影响因子：
--
作者：
H. Jarrett
通讯作者：
H. Jarrett