Schisandra chinensis acidic polysaccharide partialy reverses acetaminophen-induced liver injury in mice
Schisandra chinensis acidic polysaccharide partialy reverses acetaminophen-induced liver injury in mice
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DOI：
10.1016/j.jphs.2019.07.008
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发表时间：
2019-07-01
影响因子：
3.5
通讯作者：
Wang, Chunmei
中科院分区：
文献类型：
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作者：
Che, Jinying;Yang, Shuo;Wang, Chunmei
五味子是一种保肝草药，在中国已经使用了几个世纪。多糖是S.中国，据报道，它可以改善四氯化碳，酒精或高脂饮食引起的肝损伤。本研究观察了五味子多糖的次级成分酸性多糖（SCAP）对醋氨酚（APAP）诱导的小鼠严重急性肝损伤模型的作用及其机制。SCAP显著降低血清丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）、肿瘤坏死因子-α（TNF-α）和白细胞介素-1 β（IL-1 β）水平，并发现可减轻小鼠模型中的肝脏病理学改变。同时，SCAP对肝损伤相关酶和因子具有保护作用，如显著降低丙二醛（MDA）水平和谷胱甘肽（GSH）耗竭，降低B细胞淋巴瘤-2（Bcl-2）相关X蛋白（Bax）/Bcl-2比值，抑制caspase-3表达，增加p-AMPK、p-Akt、p-glycogen synthase kinase 3 β表达（GSK 3 β）、核因子红细胞2衍生样2（Nrf 2）和血红素加氧酶-1（HO-1）蛋白。结论：SCAP对APAP诱导的小鼠急性肝损伤的保护作用可能与其抗氧化、抗炎和抗凋亡作用有关。(C)2019作者Elsevier B. V.代表日本药理学会制作和主办。
Schisandra chinensis is a hepatoprotective herb that has been used for centuries in China. Polysaccharide is one of the major active components in S. chinensis, which has been reported to improve liver injuries induced by carbon tetrachloride, alcohol, or high-fat diet. In this study, we observed the effects and corresponding mechanisms of the secondary component of Schisandra polysaccharide (acidic polysaccharide, SCAP) on a murine model of severe acute liver injury induced by acetaminophen (APAP). SCAP significantly decreased the serum alanine aminotransferase (ALT), aspartate aminotransferas (AST), tumor necrosis factor-alpha (TNF-alpha), and interleukin-1 beta (IL-1 beta) levels, and was found to alleviate hepatic pathological alterations in the mouse model. Meanwhile, SCAP revealed a protective effects on the liver injury-related enzymes and factors, such as significantly diminished malondialdehyde (MDA) levels and glutathione (GSH) depletion, reduced ratio of B-cell lymphoma-2 (Bcl-2)-associated X protein (Bax)/Bcl-2, prohibited cleaved caspase-3 expression, and elevated the expression of p-AMPK, p-Akt, p-glycogen synthase kinase 3 beta (GSK 3 beta), nuclear factor erythroid 2ederived-like 2 (Nrf 2) and heme oxygenase-1 (HO-1) proteins in the liver tissues of the mouse model. In conclusion, we speculated that the protective activities of SCAP on the APAP-induced mouse model of acute liver injury might be related to its antioxidation, anti-inflammation and anti-apoptosis properties. (C) 2019 The Authors. Production and hosting by Elsevier B.V. on behalf of Japanese Pharmacological Society.