2-POINT CALIBRATION OF CIRCULAR DICHROMETER WITH D-10-CAMPHORSULFONIC ACID
2-POINT CALIBRATION OF CIRCULAR DICHROMETER WITH D-10-CAMPHORSULFONIC ACID
复制标题
DOI：
10.1080/00032717708067855
复制
发表时间：
1977-01-01
影响因子：
2
通讯作者：
YANG, JT
中科院分区：
文献类型：
--
作者：
CHEN, GC;YANG, JT
D-10-樟脑磺酸(CsA)的圆二色谱(CD)在290.5和192.5 nm波长处的∊L-∊R分别为2.36cm2和-4.72cm2−1。相应的摩尔椭圆度[⊝]为7,800和-15,600度cm2dmoL−1。负带和正带的比值为-2.00，提供了简单的两点校准。如果有标准的光谱偏振仪，建议在290 nm区域使用[⊝]290.5/[M]306=1.75/1.76或[⊝]290.5/([M]306−[M]270)=0.77的极值比值进行CD校准，这不受水等光学非活性杂质的存在的影响。CsA在285 nm处的摩尔吸光系数为34.5cm2−1，可用于浓度测定。这将导致(∊L−∊R)290.5/∊285=0.068或[⊝]290.5/∊285=0.068。然而，CSA必须经过提纯，以去除UV区域中具有光学活性或吸收光或两者兼有的任何杂质。
The circular dichroism (CD) of d-10-camphorsulfonic acid (CSA) in terms of ∊L- ∊Ris 2.36 and -4.72 cm2mmol−1at 290.5 and 192.5 nm, respectively. The corresponding molar ellipticities, [⊝], are 7,800 and -15,600 deg cm2dmol−1. A ratio of -2.00 for the negative and positive bands provides a simple two-point calibration. If a standardized spectropolarimeter is available, the ratio of [⊝]290.5/[M]306= 1.75 to 1.76 or [⊝]290.5/([M]306−[M]270) = 0.77 at the extrema is recommended for CD calibration at the 290 nm region, which is unaffected by the presence of optically inactive impurities such as water. Alternately, CSA has a molar absorption coefficient of 34.5 cm2mmol−1at 285 nm, which can be used for concentration determination. This leads to (∊L−∊R)290.5/∊285= 0.068 or [⊝]290.5/∊285= 226. However, CSA must be purified to remove any impurities that are optically active or absorb light or both in the UV region.